TY - JOUR
T1 - Surface modification of a porous polyurethane through a culture of human osteoblasts and an electromagnetic bioreactor
AU - Fassina, L.
AU - Visai, L.
AU - De Angelis, M. G Cusella
AU - Benazzo, F.
AU - Magenes, G.
PY - 2007
Y1 - 2007
N2 - There is increasing interest in new biomaterials and new culture methods for bone tissue engineering, in order to produce, in vitro, living constructs able to integrate in the surrounding tissue. Using an electromagnetic bioreactor (magnetic field intensity, 2 mT; frequency, 75 Hz), we investigated the effects of electromagnetic stimulation on SAOS-2 human osteoblasts seeded onto a porous polyurethane. In comparison with control conditions, the electromagnetic stimulation caused higher cell proliferation, increased surface coating with decorin and type-I collagen, and higher calcium deposition. The immunolocalization of decorin and type-I collagen showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the porous polyurethane in terms of cell colonization and coating with calcified matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.
AB - There is increasing interest in new biomaterials and new culture methods for bone tissue engineering, in order to produce, in vitro, living constructs able to integrate in the surrounding tissue. Using an electromagnetic bioreactor (magnetic field intensity, 2 mT; frequency, 75 Hz), we investigated the effects of electromagnetic stimulation on SAOS-2 human osteoblasts seeded onto a porous polyurethane. In comparison with control conditions, the electromagnetic stimulation caused higher cell proliferation, increased surface coating with decorin and type-I collagen, and higher calcium deposition. The immunolocalization of decorin and type-I collagen showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the porous polyurethane in terms of cell colonization and coating with calcified matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.
KW - Biomimetics
KW - Calcium
KW - Cell proliferation
KW - Decorin
KW - Electromagnetic stimulation
KW - Extracellular matrix
KW - Osteoblast
KW - Surface modification
KW - Type-I collagen
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M3 - Article
C2 - 17264411
AN - SCOPUS:33947507737
VL - 15
SP - 33
EP - 45
JO - Technology and Health Care
JF - Technology and Health Care
SN - 0928-7329
IS - 1
ER -