To determine the identity and functional capacity of lymphoid cells involved in allograft rejection, viable monodispersed cells, recovered from 15 rejection human renal allografts were studied by Ficoll-Isopaque gradient separation. The surface properties of these cells have been characterized and correlated with the histologic patterns of rejection. The infiltrating cells consisted of macrophages (16-28%), polymorphonuclear leukocytes (about 5%), and lymphocytes (60-80%). B lymphocytes, bearing surface Ig, and T lymphocytes (E-rosetting cells), comprised 19-51% and 31-71% of all infiltrating cells, respectively. Cells bearing Fc receptors, identified by membrane binding of heat aggregated γ-globulin, were abundant (16-55%), whereas complement receptor lymphocytes (EAC-rosetting cells) were uncommon (1-6%). The surface attributes of the infiltrating cells were compared to those of simultaneously sampled cells from recipient peripheral blood. In order to evaluate the functional capacity of lymphoid cells, a modified Rosette Inhibition Test was also performed: 14 infiltrates contained cells with a minimal inhibitory concentration of antilymphocyte globulin greater than that of corresponding blood lymphocytes. These data indicate that a heterogeneous population of mononuclear cells is present in rejected human renal allografts; moreover, the concomitant study of peripheral blood leukocytes and infiltrating cells demonstrates that the latter represent a population distinct from the circulating pool of mononuclear cells and not simply a random accumulation from blood within the graft. Finally, since as many as 55% of the cells recovered from the renal allografts bore Fc receptors, a role for antibody-dependent target-cell lysis in the rejection process is suggested.
|Number of pages||4|
|Publication status||Published - 1977|
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