CARATTERISTICHE DI SUPERFICIE E CAPACITA FUNZIONALI DEGLI INFILTRATI CELLULARI DEL TRAPIANTO RENALE

Translated title of the contribution: Surface properties and functional characteristics of cells infiltrating renal allografts

A. Cantaluppi, G. Banfi, A. Tarantino, C. Ponticelli

Research output: Contribution to journalArticle

Abstract

To determine the identity and functional capacity of lymphoid cells involved in allograft rejection, viable monodispersed cells, recovered from 15 rejection human renal allografts were studied by Ficoll-Isopaque gradient separation. The surface properties of these cells have been characterized and correlated with the histologic patterns of rejection. The infiltrating cells consisted of macrophages (16-28%), polymorphonuclear leukocytes (about 5%), and lymphocytes (60-80%). B lymphocytes, bearing surface Ig, and T lymphocytes (E-rosetting cells), comprised 19-51% and 31-71% of all infiltrating cells, respectively. Cells bearing Fc receptors, identified by membrane binding of heat aggregated γ-globulin, were abundant (16-55%), whereas complement receptor lymphocytes (EAC-rosetting cells) were uncommon (1-6%). The surface attributes of the infiltrating cells were compared to those of simultaneously sampled cells from recipient peripheral blood. In order to evaluate the functional capacity of lymphoid cells, a modified Rosette Inhibition Test was also performed: 14 infiltrates contained cells with a minimal inhibitory concentration of antilymphocyte globulin greater than that of corresponding blood lymphocytes. These data indicate that a heterogeneous population of mononuclear cells is present in rejected human renal allografts; moreover, the concomitant study of peripheral blood leukocytes and infiltrating cells demonstrates that the latter represent a population distinct from the circulating pool of mononuclear cells and not simply a random accumulation from blood within the graft. Finally, since as many as 55% of the cells recovered from the renal allografts bore Fc receptors, a role for antibody-dependent target-cell lysis in the rejection process is suggested.

Original languageItalian
Pages (from-to)181-184
Number of pages4
JournalMinerva Nefrologica
Volume24
Issue number2
Publication statusPublished - 1977

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cantaluppi, A., Banfi, G., Tarantino, A., & Ponticelli, C. (1977). CARATTERISTICHE DI SUPERFICIE E CAPACITA FUNZIONALI DEGLI INFILTRATI CELLULARI DEL TRAPIANTO RENALE. Minerva Nefrologica, 24(2), 181-184.