Surface proteomic analysis of differentiated versus stem-like osteosarcoma human cells

Marica Gemei, Claudia Corbo, Francesca D'Alessio, Rosa Di Noto, Renza Vento, Luigi Del Vecchio

Research output: Contribution to journalArticlepeer-review

Abstract

Cancer stem cell characterization represents a breakthrough in cancer research. Despite evidence showing the existence and the role of cancer stem cells in osteosarcoma (OS) onset and progression, little is known about their specific surface phenotype. To address this issue, we carried out a cytometric analysis with an antibody-array comprising 245 membrane proteins comparing the stem and differentiated OS cells. As experimental model, we chose the stem-like cell line 3aminobenzamide-OS and its parental, differentiated, cell line MG63. We identified 50 differentially expressed, 23 homogeneously expressed, and 172 not expressed proteins in the two cell line models, thus defining a surface protein signature specific for each of them. Furthermore, we selected ERK1/2 (p44/42 mitogen-activated protein kinases) as a potential pathway correlated with processes that characterize tumorigenic potential and stemness of 3aminobenzamide-OS cells.

Original languageEnglish
Pages (from-to)3293-3297
Number of pages5
JournalProteomics
Volume13
Issue number22
DOIs
Publication statusPublished - Nov 2013

Keywords

  • Cancer stem cell
  • Cell biology
  • Osteosarcoma
  • Surface proteome

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Medicine(all)

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