Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Ayush Verma, Oktay Uzun, Yuhua Hu, Ying Hu, Hee Sun Han, Nicki Watson, Suelin Chen, Darrell J. Irvine, Francesco Stellacci

Research output: Contribution to journalArticle

890 Citations (Scopus)

Abstract

Nanoscale objects are typically internalized by cells into membrane-bounded endosomes and fail to access the cytosolic cell machinery. Whereas some biomacromolecules may penetrate or fuse with cell membranes without overt membrane disruption, no synthetic material of comparable size has shown this property yet. Cationic nano-objects pass through cell membranes by generating transient holes, a process associated with cytotoxicity. Studies aimed at generating cell-penetrating nanomaterials have focused on the effect of size, shape and composition. Here, we compare membrane penetration by two nanoparticle isomers with similar composition (same hydrophobic content), one coated with subnanometre striations of alternating anionic and hydrophobic groups, and the other coated with the same moieties but in a random distribution. We show that the former particles penetrate the plasma membrane without bilayer disruption, whereas the latter are mostly trapped in endosomes. Our results offer a paradigm for analysing the fundamental problem of cell-membrane-penetrating bio- and macro-molecules.

Original languageEnglish
Pages (from-to)588-595
Number of pages8
JournalNature Materials
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Cell membranes
Monolayers
penetration
Nanoparticles
nanoparticles
membranes
striation
fuses
machinery
Membranes
cells
statistical distributions
Electric fuses
Cytotoxicity
Chemical analysis
Nanostructured materials
isomers
Isomers
Machinery
Macros

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Verma, A., Uzun, O., Hu, Y., Hu, Y., Han, H. S., Watson, N., ... Stellacci, F. (2008). Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. Nature Materials, 7(7), 588-595. https://doi.org/10.1038/nmat2202

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. / Verma, Ayush; Uzun, Oktay; Hu, Yuhua; Hu, Ying; Han, Hee Sun; Watson, Nicki; Chen, Suelin; Irvine, Darrell J.; Stellacci, Francesco.

In: Nature Materials, Vol. 7, No. 7, 07.2008, p. 588-595.

Research output: Contribution to journalArticle

Verma, A, Uzun, O, Hu, Y, Hu, Y, Han, HS, Watson, N, Chen, S, Irvine, DJ & Stellacci, F 2008, 'Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles', Nature Materials, vol. 7, no. 7, pp. 588-595. https://doi.org/10.1038/nmat2202
Verma, Ayush ; Uzun, Oktay ; Hu, Yuhua ; Hu, Ying ; Han, Hee Sun ; Watson, Nicki ; Chen, Suelin ; Irvine, Darrell J. ; Stellacci, Francesco. / Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. In: Nature Materials. 2008 ; Vol. 7, No. 7. pp. 588-595.
@article{9958b51bf19841b18f0212854d836352,
title = "Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles",
abstract = "Nanoscale objects are typically internalized by cells into membrane-bounded endosomes and fail to access the cytosolic cell machinery. Whereas some biomacromolecules may penetrate or fuse with cell membranes without overt membrane disruption, no synthetic material of comparable size has shown this property yet. Cationic nano-objects pass through cell membranes by generating transient holes, a process associated with cytotoxicity. Studies aimed at generating cell-penetrating nanomaterials have focused on the effect of size, shape and composition. Here, we compare membrane penetration by two nanoparticle isomers with similar composition (same hydrophobic content), one coated with subnanometre striations of alternating anionic and hydrophobic groups, and the other coated with the same moieties but in a random distribution. We show that the former particles penetrate the plasma membrane without bilayer disruption, whereas the latter are mostly trapped in endosomes. Our results offer a paradigm for analysing the fundamental problem of cell-membrane-penetrating bio- and macro-molecules.",
author = "Ayush Verma and Oktay Uzun and Yuhua Hu and Ying Hu and Han, {Hee Sun} and Nicki Watson and Suelin Chen and Irvine, {Darrell J.} and Francesco Stellacci",
year = "2008",
month = "7",
doi = "10.1038/nmat2202",
language = "English",
volume = "7",
pages = "588--595",
journal = "Nature Materials",
issn = "1476-1122",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

AU - Verma, Ayush

AU - Uzun, Oktay

AU - Hu, Yuhua

AU - Hu, Ying

AU - Han, Hee Sun

AU - Watson, Nicki

AU - Chen, Suelin

AU - Irvine, Darrell J.

AU - Stellacci, Francesco

PY - 2008/7

Y1 - 2008/7

N2 - Nanoscale objects are typically internalized by cells into membrane-bounded endosomes and fail to access the cytosolic cell machinery. Whereas some biomacromolecules may penetrate or fuse with cell membranes without overt membrane disruption, no synthetic material of comparable size has shown this property yet. Cationic nano-objects pass through cell membranes by generating transient holes, a process associated with cytotoxicity. Studies aimed at generating cell-penetrating nanomaterials have focused on the effect of size, shape and composition. Here, we compare membrane penetration by two nanoparticle isomers with similar composition (same hydrophobic content), one coated with subnanometre striations of alternating anionic and hydrophobic groups, and the other coated with the same moieties but in a random distribution. We show that the former particles penetrate the plasma membrane without bilayer disruption, whereas the latter are mostly trapped in endosomes. Our results offer a paradigm for analysing the fundamental problem of cell-membrane-penetrating bio- and macro-molecules.

AB - Nanoscale objects are typically internalized by cells into membrane-bounded endosomes and fail to access the cytosolic cell machinery. Whereas some biomacromolecules may penetrate or fuse with cell membranes without overt membrane disruption, no synthetic material of comparable size has shown this property yet. Cationic nano-objects pass through cell membranes by generating transient holes, a process associated with cytotoxicity. Studies aimed at generating cell-penetrating nanomaterials have focused on the effect of size, shape and composition. Here, we compare membrane penetration by two nanoparticle isomers with similar composition (same hydrophobic content), one coated with subnanometre striations of alternating anionic and hydrophobic groups, and the other coated with the same moieties but in a random distribution. We show that the former particles penetrate the plasma membrane without bilayer disruption, whereas the latter are mostly trapped in endosomes. Our results offer a paradigm for analysing the fundamental problem of cell-membrane-penetrating bio- and macro-molecules.

UR - http://www.scopus.com/inward/record.url?scp=45849144754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45849144754&partnerID=8YFLogxK

U2 - 10.1038/nmat2202

DO - 10.1038/nmat2202

M3 - Article

C2 - 18500347

AN - SCOPUS:45849144754

VL - 7

SP - 588

EP - 595

JO - Nature Materials

JF - Nature Materials

SN - 1476-1122

IS - 7

ER -