Surfactant-associated protein B kinetics in vivo in newborn infants by stable isotopes

Paola Cogo, Aldo Baritussio, Federica Rosso, Antonina Gucciardi, Vincenzo Moretti, Tamara Badon, Elena Duner, Luc Zimmernann, Virgilio P. Carnielli

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Surfactant-associated protein B (SP-B) is critical to the biophysical function of pulmonary surfactant. No information is available on SP-B synthesis and kinetics in humans. We administered a 24-h i.v. infusion of 13C-valine as metabolic precursor of SP-B to six newborn infants (weight 3.5 ± 0.5 kg; age 12 d, range 1-43 d). Three of the study infants also received i.v. 2H-palmitate to label surfactant disaturated phosphatidylcholine (DSPC). SP-B and DSPC were isolated from tracheal aspirates, and their respective 13C and 2H enrichments were measured by gas chromatography-mass spectrometry. SP-B kinetics was measured successfully in all six infants. SP-B median (range) fractional synthesis rate was 30% per day (20-78% per day), secretion time was 4.5 h (1-9 h), time to peak was 24 h (12-36 h), and half-life was 21 h (8-35 h). The ascending part of the SP-B kinetic curve was similar to the DSPC curve, suggesting similar secretion pathways. SP-B half-life seemed to be shorter than DSPC half-life. These results agree with existing animal data. We conclude that the measurement of SP-B kinetics is feasible in vivo in humans by stable isotope technology.

Original languageEnglish
Pages (from-to)519-522
Number of pages4
JournalPediatric Research
Volume57
Issue number4
DOIs
Publication statusPublished - Apr 2005

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Surface-Active Agents
Isotopes
Newborn Infant
Half-Life
IgA receptor
Pulmonary Surfactants
Palmitates
Secretory Pathway
Valine
Gas Chromatography-Mass Spectrometry
Technology
Weights and Measures
disaturated lecithin

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Cogo, P., Baritussio, A., Rosso, F., Gucciardi, A., Moretti, V., Badon, T., ... Carnielli, V. P. (2005). Surfactant-associated protein B kinetics in vivo in newborn infants by stable isotopes. Pediatric Research, 57(4), 519-522. https://doi.org/10.1203/01.PDR.0000155755.27716.04

Surfactant-associated protein B kinetics in vivo in newborn infants by stable isotopes. / Cogo, Paola; Baritussio, Aldo; Rosso, Federica; Gucciardi, Antonina; Moretti, Vincenzo; Badon, Tamara; Duner, Elena; Zimmernann, Luc; Carnielli, Virgilio P.

In: Pediatric Research, Vol. 57, No. 4, 04.2005, p. 519-522.

Research output: Contribution to journalArticle

Cogo, P, Baritussio, A, Rosso, F, Gucciardi, A, Moretti, V, Badon, T, Duner, E, Zimmernann, L & Carnielli, VP 2005, 'Surfactant-associated protein B kinetics in vivo in newborn infants by stable isotopes', Pediatric Research, vol. 57, no. 4, pp. 519-522. https://doi.org/10.1203/01.PDR.0000155755.27716.04
Cogo, Paola ; Baritussio, Aldo ; Rosso, Federica ; Gucciardi, Antonina ; Moretti, Vincenzo ; Badon, Tamara ; Duner, Elena ; Zimmernann, Luc ; Carnielli, Virgilio P. / Surfactant-associated protein B kinetics in vivo in newborn infants by stable isotopes. In: Pediatric Research. 2005 ; Vol. 57, No. 4. pp. 519-522.
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