Surfactant protein a expression in human normal and neoplastic breast epithelium

Paola Braidotti, Claudia Cigala, Daniela Graziani, Barbara Del Curto, Enrico Dessy, Guido Coggi, Silvano Bosari, Giuseppe G. Pietra

Research output: Contribution to journalArticlepeer-review


We studied the presence of surfactant protein A (Sp-A) immunoreactivity and messenger RNA in 62 normal and abnormal breast samples. Sections were immunostained with polyclonal anti-Sp-A antibody. The association between Sp-A immunoreactivity and histologic grade of 32 invasive ductal carcinomas was assessed by 3 pathologists who scored the intensity of Sp-A immunoreactivity times the percentage of tumor immunostained, individual scores were averaged, and the final scores were correlated with tumor grade, proliferative index, and expression of estrogen and progesterone receptors. Strong Sp-A immunoreactivity was present at the luminal surface of ductal epithelial cells in normal breast samples and in benign lesions; carcinomas displayed variable immunoreactivity, inversely proportional to the degree of differentiation. Sp-A messenger RNA was detected by reverse transcriptase-polymerase chain reaction in 3 of 3 normal breast sample and 9 of 9 carcinomas. The significance of Sp-A expression in breast epithelium requires further study; possibly it has a role in native host defense or epithelial differentiation.

Original languageEnglish
Pages (from-to)721-728
Number of pages8
JournalAmerican Journal of Clinical Pathology
Issue number5
Publication statusPublished - 2001


  • Breast
  • Immunohistochemistry
  • Non-immune-mediated host defense
  • PCR
  • Polymerase chain reaction
  • Sp-A
  • Surfactant protein A

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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