Surfactant protein B amount and kinetics in newborn infants: An optimized procedure

Manuela Simonato, Aldo Baritussio, Luca Vedovelli, Giulia Lamonica, Virgilio Paolo Carnielli, Paola Elisa Cogo

Research output: Contribution to journalArticle

Abstract

Surfactant protein B (SP-B) plays a key role in surfactant homeostasis affecting its biophysical properties and physiological function. Recently, a method to measure SP-B amount and kinetics from tracheal aspirates (TAs) became available. The main objective of this study was to improve the critical steps of the procedure to obtain a better SP-B sensitivity. We administered a 24 h continuous infusion of 1 mg/kg/h of 113 C-leucine to ten newborn infants. SP-B was isolated from serial TAs and its fractional synthesis rate, secretion time, peak time and half life were derived from 13 C enrichment curves obtained by gas chromatography mass spectrometry. SP-B amount in TAs was also assessed. During the extraction step, acidification and organic solvent ratio optimization doubled the recovery of SP-B from TAs, so did the elongation of the propylation time (from 20 min to 1 h) with enhanced leucine derivatization yield. Measurement of 13 C leucine enrichments, and therefore all SP-B kinetics parameters, were successfully calculated in all TAs samples due to the increase of SP-B yield. SP-B amount was 0.29 (0.16-0.41) % of total phospholipids with a minimum value of 0.08% belonging to one of the respiratory distress syndrome (RDS) patients. In conclusion, this new procedure enables accurate determination of SP-B kinetics even in the presence of low protein amount like in preterm RDS patients.

Original languageEnglish
Pages (from-to)1415-1419
Number of pages5
JournalJournal of Mass Spectrometry
Volume47
Issue number11
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Surface-Active Agents
Surface active agents
surfactants
Newborn Infant
proteins
Proteins
Kinetics
kinetics
leucine
Leucine
IgA receptor
homeostasis
secretions
Acidification
Phospholipids
gas chromatography
Kinetic parameters
half life
Gas chromatography
Gas Chromatography-Mass Spectrometry

Keywords

  • isotope
  • mass spectrometry
  • surfactant protein B

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Simonato, M., Baritussio, A., Vedovelli, L., Lamonica, G., Carnielli, V. P., & Cogo, P. E. (2012). Surfactant protein B amount and kinetics in newborn infants: An optimized procedure. Journal of Mass Spectrometry, 47(11), 1415-1419. https://doi.org/10.1002/jms.3065

Surfactant protein B amount and kinetics in newborn infants : An optimized procedure. / Simonato, Manuela; Baritussio, Aldo; Vedovelli, Luca; Lamonica, Giulia; Carnielli, Virgilio Paolo; Cogo, Paola Elisa.

In: Journal of Mass Spectrometry, Vol. 47, No. 11, 11.2012, p. 1415-1419.

Research output: Contribution to journalArticle

Simonato, M, Baritussio, A, Vedovelli, L, Lamonica, G, Carnielli, VP & Cogo, PE 2012, 'Surfactant protein B amount and kinetics in newborn infants: An optimized procedure', Journal of Mass Spectrometry, vol. 47, no. 11, pp. 1415-1419. https://doi.org/10.1002/jms.3065
Simonato M, Baritussio A, Vedovelli L, Lamonica G, Carnielli VP, Cogo PE. Surfactant protein B amount and kinetics in newborn infants: An optimized procedure. Journal of Mass Spectrometry. 2012 Nov;47(11):1415-1419. https://doi.org/10.1002/jms.3065
Simonato, Manuela ; Baritussio, Aldo ; Vedovelli, Luca ; Lamonica, Giulia ; Carnielli, Virgilio Paolo ; Cogo, Paola Elisa. / Surfactant protein B amount and kinetics in newborn infants : An optimized procedure. In: Journal of Mass Spectrometry. 2012 ; Vol. 47, No. 11. pp. 1415-1419.
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