TY - JOUR
T1 - Surfactant synthesis and kinetics in infants with congenital diaphragmatic hernia
AU - Cogo, Paola E.
AU - Zimmermann, Luc J I
AU - Rosso, Federica
AU - Tormena, Francesca
AU - Gamba, Piergiorgio
AU - Verlato, Giovanna
AU - Baritussio, Aldo
AU - Carnielli, Virgilio P.
PY - 2002/7/15
Y1 - 2002/7/15
N2 - In animal models of congenital diaphragmatic hernia (CDH), surfactant deficiency contributes to the pathophysiology of the disease; however, information on CDH in humans is limited. We compared surfactant disaturated phosphatidylcholine (DSPC) synthesis and metabolism, by stable isotope technology, in newborn infants with CDH and in control subjects. DSPC amount, total proteins, and surfactant protein-A (SP-A) from tracheal aspirates were also measured. DSPC and SP-A were significantly lower in 14 infants with CDH than in the eight control subjects. Mean DSPC was 2.3 ± 1.3 mg/ml of epithelial lining fluid (ELF) in infants with CDH and 4.6 ± 1.5 mg/ml of ELF in control subjects (p = 0.001). Mean SP-A in infants with CDH and in control subjects was 16.2 ± 9.3 and 61.2 ± 30.6 μg/ml of ELF, respectively (p = 0.03). DSPC kinetics was measured in 12 of 14 infants with CDH and in 5 of 8 control subjects. Secretion time was 8.3 ± 5.5 and 8.5 ± 2.5 hours and peak time 51.9 ± 1.5.2 and 51 ± 13 hours in infants with CDH and in control subjects, respectively. Fractional synthesis rate was not different for infants with CDH and control subjects (p = 0.4). In conclusion, surfactant DSPC synthesis and kinetics were not significantly deranged in infants with CDH compared with control subjects. Other factors, such as lower surface area or increased DSPC catabolism, may contribute to surfactant pool alteration in CDH.
AB - In animal models of congenital diaphragmatic hernia (CDH), surfactant deficiency contributes to the pathophysiology of the disease; however, information on CDH in humans is limited. We compared surfactant disaturated phosphatidylcholine (DSPC) synthesis and metabolism, by stable isotope technology, in newborn infants with CDH and in control subjects. DSPC amount, total proteins, and surfactant protein-A (SP-A) from tracheal aspirates were also measured. DSPC and SP-A were significantly lower in 14 infants with CDH than in the eight control subjects. Mean DSPC was 2.3 ± 1.3 mg/ml of epithelial lining fluid (ELF) in infants with CDH and 4.6 ± 1.5 mg/ml of ELF in control subjects (p = 0.001). Mean SP-A in infants with CDH and in control subjects was 16.2 ± 9.3 and 61.2 ± 30.6 μg/ml of ELF, respectively (p = 0.03). DSPC kinetics was measured in 12 of 14 infants with CDH and in 5 of 8 control subjects. Secretion time was 8.3 ± 5.5 and 8.5 ± 2.5 hours and peak time 51.9 ± 1.5.2 and 51 ± 13 hours in infants with CDH and in control subjects, respectively. Fractional synthesis rate was not different for infants with CDH and control subjects (p = 0.4). In conclusion, surfactant DSPC synthesis and kinetics were not significantly deranged in infants with CDH compared with control subjects. Other factors, such as lower surface area or increased DSPC catabolism, may contribute to surfactant pool alteration in CDH.
KW - Congenital diaphragmatic hernia
KW - Stable isotopes
KW - Surfactant
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U2 - 10.1164/rccm.2108028
DO - 10.1164/rccm.2108028
M3 - Article
C2 - 12119226
AN - SCOPUS:0037099342
VL - 166
SP - 154
EP - 158
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 2
ER -