Surgically-induced bile duct injury is followed by early hepatic oxidative stress. A preliminary experimental study in rats

Germana Lissidini; Giuseppe Piccinni; Piero Portincasa; Ignazio Grattagliano; Angela Gurrado; Mario Testini

Surgically-induced bile duct injury is followed by early hepatic oxidative stress. A preliminary experimental study in rats

Germana Lissidini, Giuseppe Piccinni, Piero Portincasa, Ignazio Grattagliano, Angela Gurrado, Mario Testini

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article › peer-review

Abstract

Background/Aims: To study in the rat whether oxidative stress appears early in a model of short-term cholestasis induced by bile duct ligation. Methodology: Adult male rats underwent proximal bile duct ligation; controls underwent a sham operation. Animals were sacrificed at days 0, 1, 3, 7 and 10. Livers were removed for histological studies and biochemical analysis. Results: GSH and PSH levels declined earlier in mitochondria than cytosol. Data were analyzed for statistical significance by ANOVA analysis. Conclusions: This study represents an experimental model which helps in the interpretation of the damage induced by bile duct injury in humans emphasizing an early detection of bile duct injury after surgery to ensure appropriate treatment and optimal patient outcome.

Original language	English
Pages (from-to)	602-605
Number of pages	4
Journal	Hepato-Gastroenterology
Volume	56
Issue number	91-92
Publication status	Published - May 2009

Keywords

Bile duct injury
Cholestasis
Hepatobiliary surgery

ASJC Scopus subject areas

Gastroenterology
Hepatology

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Surgically-induced bile duct injury is followed by early hepatic oxidative stress. A preliminary experimental study in rats. / Lissidini, Germana; Piccinni, Giuseppe; Portincasa, Piero; Grattagliano, Ignazio; Gurrado, Angela; Testini, Mario.

In: Hepato-Gastroenterology, Vol. 56, No. 91-92, 05.2009, p. 602-605.

Research output: Contribution to journal › Article › peer-review

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AU - Grattagliano, Ignazio

AU - Gurrado, Angela

AU - Testini, Mario

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KW - Cholestasis

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Surgically-induced bile duct injury is followed by early hepatic oxidative stress. A preliminary experimental study in rats

Abstract

Keywords

ASJC Scopus subject areas

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