Abstract
One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of B. pertussis. The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European B. pertussis population is changing and became more homogenous after the introduction of acellular pertussis vaccines.
| Original language | English |
|---|---|
| Article number | e01998-17 |
| Journal | Journal of Clinical Microbiology |
| Volume | 56 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1 2018 |
Fingerprint
Keywords
- Bordetella pertussis
- Europe
- Genotyping
- MLVA
- PFGE
- Serotyping
ASJC Scopus subject areas
- Microbiology (medical)
Cite this
Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015. / Barkoff, Alex Mikael; Mertsola, Jussi; Pierard, Denis; Dalby, Tine; Hoegh, Silje Vermedal; Guillot, Sophie; Stefanelli, Paola; Van Gent, Marjolein; Berbers, Guy; Vestrheim, Didrik F.; Greve-Isdahl, Margrethe; Wehlin, Lena; Ljungman, Margaretha; Fry, Norman K.; Markey, Kevin; Auranen, Kari; Hea, Qiushui.
In: Journal of Clinical Microbiology, Vol. 56, No. 5, e01998-17, 01.05.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015
AU - Barkoff, Alex Mikael
AU - Mertsola, Jussi
AU - Pierard, Denis
AU - Dalby, Tine
AU - Hoegh, Silje Vermedal
AU - Guillot, Sophie
AU - Stefanelli, Paola
AU - Van Gent, Marjolein
AU - Berbers, Guy
AU - Vestrheim, Didrik F.
AU - Greve-Isdahl, Margrethe
AU - Wehlin, Lena
AU - Ljungman, Margaretha
AU - Fry, Norman K.
AU - Markey, Kevin
AU - Auranen, Kari
AU - Hea, Qiushui
PY - 2018/5/1
Y1 - 2018/5/1
N2 - One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of B. pertussis. The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European B. pertussis population is changing and became more homogenous after the introduction of acellular pertussis vaccines.
AB - One reason for increased pertussis incidence is the adaptation of Bordetella pertussis to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (n 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of B. pertussis. The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European B. pertussis population is changing and became more homogenous after the introduction of acellular pertussis vaccines.
KW - Bordetella pertussis
KW - Europe
KW - Genotyping
KW - MLVA
KW - PFGE
KW - Serotyping
UR - http://www.scopus.com/inward/record.url?scp=85046255980&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046255980&partnerID=8YFLogxK
U2 - 10.1128/JCM.01998-17
DO - 10.1128/JCM.01998-17
M3 - Article
C2 - 29491017
AN - SCOPUS:85046255980
VL - 56
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 5
M1 - e01998-17
ER -