Background. The islet autoimmune process that characterizes the development of type 1 diabetes mellitus and latent autoimmune diabetes in adults is made evident by the appearance of circulating autoantibodies directed against glutamic acid decarboxylase (GADA), insulin (IAA), protein-tyrosin-phosphatase IA-2 (IA-2A) and zinc transporter ZnT8 (ZnT8A). The diffusion of methods for the determination of islet autoantibodies in Italian Laboratories is not known. Methods. A questionnaire on methods used for the determination of ICA was sent to Italian clinical Laboratories. Results. 61 questionnaires have been collected; 41 Laboratories declared that they performed routinely the analyses of islet autoantibodies or received serum samples that were sent to other Laboratories to perform such determinations. GADA were analysed in 30 Laboratories, ICA in 26 Laboratories, IA-2A in 17 Laboratories and IAA in 16 Laboratories. One Laboratory performed also ZnT8A for clinical use. Radioimmunological methods were used by 61% of Laboratories that analysed GADA, 75% of Laboratories that analysed IA-2A and 65% of Laboratories that analysed IAA. The other Laboratories used ELISAs. ICA were determined using indirect immunofluorescence in 96% of Laboratories, but criostatic sections of human pancreas were used only by 17% of Laboratories. Conclusions. The determinations of islet autoantibodies are routinely performed by a large number of clinical Laboratories. GADA analysis is the most widely performed determination. A large number of Laboratories is still routinely performing the ICA analysis, by using the indirect immunofluorescence technique. In 6 Laboratories, ICA is the only islet autoantibody assay available. High sensitivity and specificity radioimmunological methods are used by approximately 60-75% of Laboratories.
|Translated title of the contribution||Survey on the determination of islet autoantibodies in Italian Laboratories|
|Number of pages||8|
|Journal||Rivista Italiana della Medicina di Laboratorio|
|Publication status||Published - 2014|
ASJC Scopus subject areas
- Biochemistry, medical
- Medical Laboratory Technology