Survivin expression in "low-risk" and "high-risk" myelodysplastic syndromes

Umberto Gianelli, Nicola Stefano Fracchiolla, Agostino Cortelezzi, Caterina Pellegrini, Federica Savi, Alessia Moro, Maria Grazia Grimoldi, Giorgio Lambertenghi Deliliers, Guido Coggi, Silvano Bosari

Research output: Contribution to journalArticlepeer-review

Abstract

Apoptosis has a crucial role in myelodysplastic syndromes (MDS), being responsible of the ineffective hematopoiesis characteristic of the disease. Apoptosis rate is elevated in "early phase" MDS, whereas it diminishes during disease progression to acute leukemia, consensually to the acquisition of independent growth features. Survivin is a member of the inhibitor of the apoptosis (IAP) family, with the bifunctional role of suppressing apoptosis while facilitating cell cycle progression. We investigated Survivin mRNA levels by real-time quantitative reverse transcriptase PCR analysis and Survivin protein expression by immunohistochemistry in 49 bone marrow (BM) aspirates and in 17 BM biopsies (BMB) from MDS patients. Survivin mRNA levels were higher in MDS than in control group (1.68 ± 1.46 vs 0.25 ± 0.22; p > 0.0001). MDS patients with low or INT1 International Scoring System for Evaluating Prognosis (IPSS) displayed higher levels of Survivin mRNA in comparison to INT2 or high IPSS (1.91 ± 1.51 vs 0.88 ± 0.95; p = 0.0058). Survivin protein immunoreactivity was evaluated as Survivin index S (i) and calculated according to the formula: S (i) = % of Survivin positive cells × BMB cellularity/100. Survivin index was higher in the MDS group than in normal BM (p = 0.05). Moreover, in eight cases in which BM aspirates and trephine biopsy were available, we found a significant association between the level of Survivin mRNA and protein expression (p = 0.011). In conclusion, this study demonstrates increased levels of Survivin in MDS compared to normal controls. Moreover, higher levels of transcripts are related to "low-risk" MDS. Our results suggest an active role of Survivin in normal and in myelodysplastic hematopoiesis.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalAnnals of Hematology
Volume86
Issue number3
DOIs
Publication statusPublished - Mar 2007

Keywords

  • Immunohistochemistry
  • Myelodysplastic syndromes
  • Real-time RT-PCR
  • Survivin

ASJC Scopus subject areas

  • Hematology

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