Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma

Salvatore De Maria, Giuseppe Pannone, Pantaleo Bufo, Angela Santoro, Rosario Serpico, Salvatore Metafora, Corrado Rubini, Daniela Pasquali, Silvana M. Papagerakis, Stefania Staibano, Gaetano De Rosa, Ernesto Farina, Monica Emanuelli, Andrea Santarelli, Maria Ada Mariggiò, Lucio Lo Russo, Lorenzo Lo Muzio

Research output: Contribution to journalArticle

Abstract

Purpose: Survivin, an inhibitor of apoptosis protein and a cell cycle regulator, has been detected in the majority of human cancers. Five splice variants (survivin, survivin-2α, survivin-2B, survivin-3B, and survivin-ΔEx3) have been identified; their expressions have been investigated here. Methods: By means of RT real-time PCR and immunohistochemistry, we have evaluated survivin isoform expressions at both mRNA and protein levels in human normal oral tissue, precancerous lesions, and oral squamous cell carcinoma (OSCC). Their correlations with the pathological findings have also been analyzed. Results: Expression levels of all survivin transcript variants were markedly elevated in OSCC when compared to normal tissues. One-way analysis of variance (ANOVA) revealed highly significant up-regulation of survivin (P = 0.001), survivin-ΔEx3 (P = 0.001) and survivin-2B (P = 0.004), whereas survivin-3B showed a minor increase in OSCC compared to normal mucosa. Conclusions: Our findings suggest that survivin isoforms deregulation may have significant implications in tumor aggressiveness and prognosis.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalJournal of Cancer Research and Clinical Oncology
Volume135
Issue number1
DOIs
Publication statusPublished - Jan 2009

Keywords

  • Apoptosis
  • Dysplasia
  • OSCC
  • Splicing isoforms
  • Survivin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    De Maria, S., Pannone, G., Bufo, P., Santoro, A., Serpico, R., Metafora, S., Rubini, C., Pasquali, D., Papagerakis, S. M., Staibano, S., De Rosa, G., Farina, E., Emanuelli, M., Santarelli, A., Mariggiò, M. A., Lo Russo, L., & Lo Muzio, L. (2009). Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma. Journal of Cancer Research and Clinical Oncology, 135(1), 107-116. https://doi.org/10.1007/s00432-008-0433-z