Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma

Salvatore De Maria, Giuseppe Pannone, Pantaleo Bufo, Angela Santoro, Rosario Serpico, Salvatore Metafora, Corrado Rubini, Daniela Pasquali, Silvana M. Papagerakis, Stefania Staibano, Gaetano De Rosa, Ernesto Farina, Monica Emanuelli, Andrea Santarelli, Maria Ada Mariggiò, Lucio Lo Russo, Lorenzo Lo Muzio

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Purpose: Survivin, an inhibitor of apoptosis protein and a cell cycle regulator, has been detected in the majority of human cancers. Five splice variants (survivin, survivin-2α, survivin-2B, survivin-3B, and survivin-ΔEx3) have been identified; their expressions have been investigated here. Methods: By means of RT real-time PCR and immunohistochemistry, we have evaluated survivin isoform expressions at both mRNA and protein levels in human normal oral tissue, precancerous lesions, and oral squamous cell carcinoma (OSCC). Their correlations with the pathological findings have also been analyzed. Results: Expression levels of all survivin transcript variants were markedly elevated in OSCC when compared to normal tissues. One-way analysis of variance (ANOVA) revealed highly significant up-regulation of survivin (P = 0.001), survivin-ΔEx3 (P = 0.001) and survivin-2B (P = 0.004), whereas survivin-3B showed a minor increase in OSCC compared to normal mucosa. Conclusions: Our findings suggest that survivin isoforms deregulation may have significant implications in tumor aggressiveness and prognosis.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalJournal of Cancer Research and Clinical Oncology
Volume135
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Squamous Cell Carcinoma
Protein Isoforms
Gene Expression
Inhibitor of Apoptosis Proteins
Real-Time Polymerase Chain Reaction
Neoplasms
Analysis of Variance
Cell Cycle
Mucous Membrane
Up-Regulation
Immunohistochemistry
Messenger RNA
Proteins

Keywords

  • Apoptosis
  • Dysplasia
  • OSCC
  • Splicing isoforms
  • Survivin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

De Maria, S., Pannone, G., Bufo, P., Santoro, A., Serpico, R., Metafora, S., ... Lo Muzio, L. (2009). Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma. Journal of Cancer Research and Clinical Oncology, 135(1), 107-116. https://doi.org/10.1007/s00432-008-0433-z

Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma. / De Maria, Salvatore; Pannone, Giuseppe; Bufo, Pantaleo; Santoro, Angela; Serpico, Rosario; Metafora, Salvatore; Rubini, Corrado; Pasquali, Daniela; Papagerakis, Silvana M.; Staibano, Stefania; De Rosa, Gaetano; Farina, Ernesto; Emanuelli, Monica; Santarelli, Andrea; Mariggiò, Maria Ada; Lo Russo, Lucio; Lo Muzio, Lorenzo.

In: Journal of Cancer Research and Clinical Oncology, Vol. 135, No. 1, 01.2009, p. 107-116.

Research output: Contribution to journalArticle

De Maria, S, Pannone, G, Bufo, P, Santoro, A, Serpico, R, Metafora, S, Rubini, C, Pasquali, D, Papagerakis, SM, Staibano, S, De Rosa, G, Farina, E, Emanuelli, M, Santarelli, A, Mariggiò, MA, Lo Russo, L & Lo Muzio, L 2009, 'Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma', Journal of Cancer Research and Clinical Oncology, vol. 135, no. 1, pp. 107-116. https://doi.org/10.1007/s00432-008-0433-z
De Maria, Salvatore ; Pannone, Giuseppe ; Bufo, Pantaleo ; Santoro, Angela ; Serpico, Rosario ; Metafora, Salvatore ; Rubini, Corrado ; Pasquali, Daniela ; Papagerakis, Silvana M. ; Staibano, Stefania ; De Rosa, Gaetano ; Farina, Ernesto ; Emanuelli, Monica ; Santarelli, Andrea ; Mariggiò, Maria Ada ; Lo Russo, Lucio ; Lo Muzio, Lorenzo. / Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma. In: Journal of Cancer Research and Clinical Oncology. 2009 ; Vol. 135, No. 1. pp. 107-116.
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abstract = "Purpose: Survivin, an inhibitor of apoptosis protein and a cell cycle regulator, has been detected in the majority of human cancers. Five splice variants (survivin, survivin-2α, survivin-2B, survivin-3B, and survivin-ΔEx3) have been identified; their expressions have been investigated here. Methods: By means of RT real-time PCR and immunohistochemistry, we have evaluated survivin isoform expressions at both mRNA and protein levels in human normal oral tissue, precancerous lesions, and oral squamous cell carcinoma (OSCC). Their correlations with the pathological findings have also been analyzed. Results: Expression levels of all survivin transcript variants were markedly elevated in OSCC when compared to normal tissues. One-way analysis of variance (ANOVA) revealed highly significant up-regulation of survivin (P = 0.001), survivin-ΔEx3 (P = 0.001) and survivin-2B (P = 0.004), whereas survivin-3B showed a minor increase in OSCC compared to normal mucosa. Conclusions: Our findings suggest that survivin isoforms deregulation may have significant implications in tumor aggressiveness and prognosis.",
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AU - Pannone, Giuseppe

AU - Bufo, Pantaleo

AU - Santoro, Angela

AU - Serpico, Rosario

AU - Metafora, Salvatore

AU - Rubini, Corrado

AU - Pasquali, Daniela

AU - Papagerakis, Silvana M.

AU - Staibano, Stefania

AU - De Rosa, Gaetano

AU - Farina, Ernesto

AU - Emanuelli, Monica

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AU - Mariggiò, Maria Ada

AU - Lo Russo, Lucio

AU - Lo Muzio, Lorenzo

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N2 - Purpose: Survivin, an inhibitor of apoptosis protein and a cell cycle regulator, has been detected in the majority of human cancers. Five splice variants (survivin, survivin-2α, survivin-2B, survivin-3B, and survivin-ΔEx3) have been identified; their expressions have been investigated here. Methods: By means of RT real-time PCR and immunohistochemistry, we have evaluated survivin isoform expressions at both mRNA and protein levels in human normal oral tissue, precancerous lesions, and oral squamous cell carcinoma (OSCC). Their correlations with the pathological findings have also been analyzed. Results: Expression levels of all survivin transcript variants were markedly elevated in OSCC when compared to normal tissues. One-way analysis of variance (ANOVA) revealed highly significant up-regulation of survivin (P = 0.001), survivin-ΔEx3 (P = 0.001) and survivin-2B (P = 0.004), whereas survivin-3B showed a minor increase in OSCC compared to normal mucosa. Conclusions: Our findings suggest that survivin isoforms deregulation may have significant implications in tumor aggressiveness and prognosis.

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