Survivin is highly expressed and promotes cell survival in malignant peritoneal mesothelioma

Nadia Zaffaroni, Aurora Costa, Marzia Pennati, Cinzia De Marco, Emanuela Affini, Maria Madeo, Roberta Erdas, Antonello Cabras, Shigeki Kusamura, Dario Baratti, Marcello Deraco, Maria Grazia Daidone

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Background: The biology of malignant peritoneal mesothelioma (MPM) is largely unknown. In the present study, we assessed the expression of survivin and other members of the inhibitors of apoptosis proteins (IAP) family (IAP-1, IAP-2 and X-IAP) in a series of 32 MPM surgical specimens and investigated the effects of survivin knockdown in an established MPM cell line. Methods: Expression of different IAPs was measured by immunohistochemistry. MPM cells were transfected with a small-interfering RNA (siRNA) targeting survivin mRNA and analyzed for survivin expression, growth rate, and ability to undergo spontaneous and drug (cisplatin, doxorubicin)-induced apoptosis. Results: Survivin expression was observed in 29 (91%) surgical MPM specimens, whereas the positivity rate for the other IAPs ranged from 69% to 100%. Transfection of MPM cells with the survivin siRNA induced a marked inhibition of survivin protein expression, a time-dependent decline in cell growth and an enhanced rate of spontaneous and drug-induced apoptosis, with a concomitant increase in the catalytic activity of caspase-9. Conclusion: Our results show for the first time that survivin, as well as other IAPs, is largely expressed in clinical MPMs and suggest that strategies aimed at down-regulating survivin may provide a novel approach for the treatment of the malignancy.

Original languageEnglish
Pages (from-to)453-466
Number of pages14
JournalCellular Oncology
Volume29
Issue number6
Publication statusPublished - 2007

Fingerprint

Inhibitor of Apoptosis Proteins
Cell Survival
Small Interfering RNA
Apoptosis
Caspase 9
Growth
Pharmaceutical Preparations
Doxorubicin
Cisplatin
Transfection
Malignant Mesothelioma
Immunohistochemistry
Cell Line
Messenger RNA
Neoplasms
Proteins

Keywords

  • Apoptosis
  • Caspase-9
  • IAPs
  • Peritoneal mesothelioma
  • siRNA
  • Survivin

ASJC Scopus subject areas

  • Cell Biology
  • Pathology and Forensic Medicine
  • Oncology

Cite this

Survivin is highly expressed and promotes cell survival in malignant peritoneal mesothelioma. / Zaffaroni, Nadia; Costa, Aurora; Pennati, Marzia; De Marco, Cinzia; Affini, Emanuela; Madeo, Maria; Erdas, Roberta; Cabras, Antonello; Kusamura, Shigeki; Baratti, Dario; Deraco, Marcello; Daidone, Maria Grazia.

In: Cellular Oncology, Vol. 29, No. 6, 2007, p. 453-466.

Research output: Contribution to journalArticle

Zaffaroni, N, Costa, A, Pennati, M, De Marco, C, Affini, E, Madeo, M, Erdas, R, Cabras, A, Kusamura, S, Baratti, D, Deraco, M & Daidone, MG 2007, 'Survivin is highly expressed and promotes cell survival in malignant peritoneal mesothelioma', Cellular Oncology, vol. 29, no. 6, pp. 453-466.
Zaffaroni N, Costa A, Pennati M, De Marco C, Affini E, Madeo M et al. Survivin is highly expressed and promotes cell survival in malignant peritoneal mesothelioma. Cellular Oncology. 2007;29(6):453-466.
Zaffaroni, Nadia ; Costa, Aurora ; Pennati, Marzia ; De Marco, Cinzia ; Affini, Emanuela ; Madeo, Maria ; Erdas, Roberta ; Cabras, Antonello ; Kusamura, Shigeki ; Baratti, Dario ; Deraco, Marcello ; Daidone, Maria Grazia. / Survivin is highly expressed and promotes cell survival in malignant peritoneal mesothelioma. In: Cellular Oncology. 2007 ; Vol. 29, No. 6. pp. 453-466.
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AU - Costa, Aurora

AU - Pennati, Marzia

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AU - Affini, Emanuela

AU - Madeo, Maria

AU - Erdas, Roberta

AU - Cabras, Antonello

AU - Kusamura, Shigeki

AU - Baratti, Dario

AU - Deraco, Marcello

AU - Daidone, Maria Grazia

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