Susceptibility of Human Placenta Derived Mesenchymal Stromal/Stem Cells to Human Herpesviruses Infection

Simone Avanzi, Valerio Leoni, Antonella Rotola, Francesco Alviano, Liliana Solimando, Giacomo Lanzoni, Laura Bonsi, Dario Di Luca, Cosetta Marchionni, Gualtiero Alvisi, Alessandro Ripalti

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fetal membranes (FM) derived mesenchymal stromal/stem cells (MSCs) are higher in number, expansion and differentiation abilities compared with those obtained from adult tissues, including bone marrow. Upon systemic administration, ex vivo expanded FM-MSCs preferentially home to damaged tissues promoting regenerative processes through their unique biological properties. These characteristics together with their immune-privileged nature and immune suppressive activity, a low infection rate and young age of placenta compared to other sources of SCs make FM-MSCs an attractive target for cell-based therapy and a valuable tool in regenerative medicine, currently being evaluated in clinical trials. In the present study we investigated the permissivity of FM-MSCs to all members of the human Herpesviridae family, an issue which is relevant to their purification, propagation, conservation and therapeutic use, as well as to their potential role in the vertical transmission of viral agents to the fetus and to their potential viral vector-mediated genetic modification. We present here evidence that FM-MSCs are fully permissive to infection with Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), Varicella zoster virus (VZV), and Human Cytomegalovirus (HCMV), but not with Epstein-Barr virus (EBV), Human Herpesvirus-6, 7 and 8 (HHV-6, 7, 8) although these viruses are capable of entering FM-MSCs and transient, limited viral gene expression occurs. Our findings therefore strongly suggest that FM-MSCs should be screened for the presence of herpesviruses before xenotransplantation. In addition, they suggest that herpesviruses may be indicated as viral vectors for gene expression in MSCs both in gene therapy applications and in the selective induction of differentiation.

Original languageEnglish
Article numbere71412
JournalPLoS One
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 5 2013

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Herpesviridae Infections
stromal cells
extraembryonic membranes
Stem cells
Mesenchymal Stromal Cells
placenta
Placenta
stem cells
Membranes
Extraembryonic Membranes
Viruses
Human herpesvirus 6
Gene expression
Herpesviridae
Human Herpesvirus 7
Human herpesvirus 3
Human herpesvirus 2
Human herpesvirus 5
Tissue
Human Herpesvirus 6

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Avanzi, S., Leoni, V., Rotola, A., Alviano, F., Solimando, L., Lanzoni, G., ... Ripalti, A. (2013). Susceptibility of Human Placenta Derived Mesenchymal Stromal/Stem Cells to Human Herpesviruses Infection. PLoS One, 8(8), [e71412]. https://doi.org/10.1371/journal.pone.0071412

Susceptibility of Human Placenta Derived Mesenchymal Stromal/Stem Cells to Human Herpesviruses Infection. / Avanzi, Simone; Leoni, Valerio; Rotola, Antonella; Alviano, Francesco; Solimando, Liliana; Lanzoni, Giacomo; Bonsi, Laura; Di Luca, Dario; Marchionni, Cosetta; Alvisi, Gualtiero; Ripalti, Alessandro.

In: PLoS One, Vol. 8, No. 8, e71412, 05.08.2013.

Research output: Contribution to journalArticle

Avanzi, S, Leoni, V, Rotola, A, Alviano, F, Solimando, L, Lanzoni, G, Bonsi, L, Di Luca, D, Marchionni, C, Alvisi, G & Ripalti, A 2013, 'Susceptibility of Human Placenta Derived Mesenchymal Stromal/Stem Cells to Human Herpesviruses Infection', PLoS One, vol. 8, no. 8, e71412. https://doi.org/10.1371/journal.pone.0071412
Avanzi, Simone ; Leoni, Valerio ; Rotola, Antonella ; Alviano, Francesco ; Solimando, Liliana ; Lanzoni, Giacomo ; Bonsi, Laura ; Di Luca, Dario ; Marchionni, Cosetta ; Alvisi, Gualtiero ; Ripalti, Alessandro. / Susceptibility of Human Placenta Derived Mesenchymal Stromal/Stem Cells to Human Herpesviruses Infection. In: PLoS One. 2013 ; Vol. 8, No. 8.
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