Sympathectomy alters bone architecture in adult growing rats

F. Pagani, V. Sibilia, F. Cavani, M. Ferretti, L. Bertoni, C. Palumbo, N. Lattuada, E. De Luca, A. Rubinacci, F. Guidobono

Research output: Contribution to journalArticlepeer-review


Sympathetic nervous system (SNS) fibres and α- and β-receptors are present in bone, indicating that the SNS may participate in bone metabolism. The importance of these observations is controversial because stimulation or inhibition of the SNS has had various effects upon both anabolic and catabolic activity in this tissue. In this study we evaluated the effects of pharmacological sympathectomy, using chronic treatment of maturing male rats with 40 mg of guanethidine/kg i.p., upon various parameters in bone. Double labelling with tetracycline injection was also performed 20 and 2 days before sacrifice. Bone mass, mineral content, density and histomorphometric characteristics in different skeletal regions were determined. Bone metabolic markers included urinary deoxypyridinoline and serum osteocalcin measurements. Guanethidine significantly reduced the accretion of lumbar vertebral bone and of mineral content and density, compared to controls. Femoral bone mineral content and density were also significantly reduced, compared to controls. Histomorphometric analyses indicated these effects were related to a reduction of cortical bone and mineral apposition rate at femoral diaphysial level. Both markers of bone metabolism were reduced in controls as they approached maturity. Guanethidine significantly decreased serum osteocalcin compared to controls, while urinary deoxypyridinoline was unchanged. These data indicate that guanethidine-induced sympathectomy caused a negative balance of bone metabolism, leading to decreased mass by regulating deposition rather than resorption during modeling and remodeling of bone.

Original languageEnglish
Pages (from-to)2155-2164
Number of pages10
JournalJournal of Cellular Biochemistry
Issue number6
Publication statusPublished - Aug 15 2008


  • Bone histomorphometry
  • Bone mineral density
  • Deoxypyridinoline
  • DXA
  • Guanethidine
  • Osteocalcin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology


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