Synaptic and memory dysfunction induced by tau oligomers is rescued by up-regulation of the nitric oxide cascade

Erica Acquarone, Elentina K. Argyrousi, Manon Van Den Berg, Walter Gulisano, Mauro Fà, Agnieszka Staniszewski, Elisa Calcagno, Elisa Zuccarello, Luciano D'Adamio, Shi Xian Deng, Daniela Puzzo, Ottavio Arancio, Jole Fiorito

Research output: Contribution to journalArticle

Abstract

Background: Soluble aggregates of oligomeric forms of tau protein (oTau) have been associated with impairment of synaptic plasticity and memory in Alzheimer's disease. However, the molecular mechanisms underlying the synaptic and memory dysfunction induced by elevation of oTau are still unknown. Methods: This work used a combination of biochemical, electrophysiological and behavioral techniques. Biochemical methods included analysis of phosphorylation of the cAMP-responsive element binding (CREB) protein, a transcriptional factor involved in memory, histone acetylation, and expression immediate early genes c-Fos and Arc. Electrophysiological methods included assessment of long-term potentiation (LTP), a type of synaptic plasticity thought to underlie memory formation. Behavioral studies investigated both short-term spatial memory and associative memory. These phenomena were examined following oTau elevation. Results: Levels of phospho-CREB, histone 3 acetylation at lysine 27, and immediate early genes c-Fos and Arc, were found to be reduced after oTau elevation during memory formation. These findings led us to explore whether up-regulation of various components of the nitric oxide (NO) signaling pathway impinging onto CREB is capable of rescuing oTau-induced impairment of plasticity, memory, and CREB phosphorylation. The increase of NO levels protected against oTau-induced impairment of LTP through activation of soluble guanylyl cyclase. Similarly, the elevation of cGMP levels and stimulation of the cGMP-dependent protein kinases (PKG) re-established normal LTP after exposure to oTau. Pharmacological inhibition of cGMP degradation through inhibition of phosphodiesterase 5 (PDE5), rescued oTau-induced LTP reduction. These findings could be extrapolated to memory because PKG activation and PDE5 inhibition rescued oTau-induced memory impairment. Finally, PDE5 inhibition re-established normal elevation of CREB phosphorylation and cGMP levels after memory induction in the presence of oTau. Conclusions: Up-regulation of CREB activation through agents acting on the NO cascade might be beneficial against tau-induced synaptic and memory dysfunctions.

Original languageEnglish
Article number26
JournalMolecular Neurodegeneration
Volume14
Issue number1
DOIs
Publication statusPublished - Jun 27 2019

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Nitric Oxide
Up-Regulation
Long-Term Potentiation
Type 5 Cyclic Nucleotide Phosphodiesterases
Neuronal Plasticity
Immediate-Early Genes
Phosphorylation
Acetylation
Histones
Cyclic GMP-Dependent Protein Kinases
tau Proteins
Short-Term Memory
Lysine
Carrier Proteins
Alzheimer Disease
Pharmacology
Inhibition (Psychology)

Keywords

  • Alzheimer's disease
  • CREB
  • Memory
  • Nitric oxide
  • PDE5
  • Protein kinase G
  • Soluble guanylyl cyclase
  • Tau oligomers

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Acquarone, E., Argyrousi, E. K., Van Den Berg, M., Gulisano, W., Fà, M., Staniszewski, A., ... Fiorito, J. (2019). Synaptic and memory dysfunction induced by tau oligomers is rescued by up-regulation of the nitric oxide cascade. Molecular Neurodegeneration, 14(1), [26]. https://doi.org/10.1186/s13024-019-0326-4

Synaptic and memory dysfunction induced by tau oligomers is rescued by up-regulation of the nitric oxide cascade. / Acquarone, Erica; Argyrousi, Elentina K.; Van Den Berg, Manon; Gulisano, Walter; Fà, Mauro; Staniszewski, Agnieszka; Calcagno, Elisa; Zuccarello, Elisa; D'Adamio, Luciano; Deng, Shi Xian; Puzzo, Daniela; Arancio, Ottavio; Fiorito, Jole.

In: Molecular Neurodegeneration, Vol. 14, No. 1, 26, 27.06.2019.

Research output: Contribution to journalArticle

Acquarone, E, Argyrousi, EK, Van Den Berg, M, Gulisano, W, Fà, M, Staniszewski, A, Calcagno, E, Zuccarello, E, D'Adamio, L, Deng, SX, Puzzo, D, Arancio, O & Fiorito, J 2019, 'Synaptic and memory dysfunction induced by tau oligomers is rescued by up-regulation of the nitric oxide cascade', Molecular Neurodegeneration, vol. 14, no. 1, 26. https://doi.org/10.1186/s13024-019-0326-4
Acquarone, Erica ; Argyrousi, Elentina K. ; Van Den Berg, Manon ; Gulisano, Walter ; Fà, Mauro ; Staniszewski, Agnieszka ; Calcagno, Elisa ; Zuccarello, Elisa ; D'Adamio, Luciano ; Deng, Shi Xian ; Puzzo, Daniela ; Arancio, Ottavio ; Fiorito, Jole. / Synaptic and memory dysfunction induced by tau oligomers is rescued by up-regulation of the nitric oxide cascade. In: Molecular Neurodegeneration. 2019 ; Vol. 14, No. 1.
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AU - Argyrousi, Elentina K.

AU - Van Den Berg, Manon

AU - Gulisano, Walter

AU - Fà, Mauro

AU - Staniszewski, Agnieszka

AU - Calcagno, Elisa

AU - Zuccarello, Elisa

AU - D'Adamio, Luciano

AU - Deng, Shi Xian

AU - Puzzo, Daniela

AU - Arancio, Ottavio

AU - Fiorito, Jole

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N2 - Background: Soluble aggregates of oligomeric forms of tau protein (oTau) have been associated with impairment of synaptic plasticity and memory in Alzheimer's disease. However, the molecular mechanisms underlying the synaptic and memory dysfunction induced by elevation of oTau are still unknown. Methods: This work used a combination of biochemical, electrophysiological and behavioral techniques. Biochemical methods included analysis of phosphorylation of the cAMP-responsive element binding (CREB) protein, a transcriptional factor involved in memory, histone acetylation, and expression immediate early genes c-Fos and Arc. Electrophysiological methods included assessment of long-term potentiation (LTP), a type of synaptic plasticity thought to underlie memory formation. Behavioral studies investigated both short-term spatial memory and associative memory. These phenomena were examined following oTau elevation. Results: Levels of phospho-CREB, histone 3 acetylation at lysine 27, and immediate early genes c-Fos and Arc, were found to be reduced after oTau elevation during memory formation. These findings led us to explore whether up-regulation of various components of the nitric oxide (NO) signaling pathway impinging onto CREB is capable of rescuing oTau-induced impairment of plasticity, memory, and CREB phosphorylation. The increase of NO levels protected against oTau-induced impairment of LTP through activation of soluble guanylyl cyclase. Similarly, the elevation of cGMP levels and stimulation of the cGMP-dependent protein kinases (PKG) re-established normal LTP after exposure to oTau. Pharmacological inhibition of cGMP degradation through inhibition of phosphodiesterase 5 (PDE5), rescued oTau-induced LTP reduction. These findings could be extrapolated to memory because PKG activation and PDE5 inhibition rescued oTau-induced memory impairment. Finally, PDE5 inhibition re-established normal elevation of CREB phosphorylation and cGMP levels after memory induction in the presence of oTau. Conclusions: Up-regulation of CREB activation through agents acting on the NO cascade might be beneficial against tau-induced synaptic and memory dysfunctions.

AB - Background: Soluble aggregates of oligomeric forms of tau protein (oTau) have been associated with impairment of synaptic plasticity and memory in Alzheimer's disease. However, the molecular mechanisms underlying the synaptic and memory dysfunction induced by elevation of oTau are still unknown. Methods: This work used a combination of biochemical, electrophysiological and behavioral techniques. Biochemical methods included analysis of phosphorylation of the cAMP-responsive element binding (CREB) protein, a transcriptional factor involved in memory, histone acetylation, and expression immediate early genes c-Fos and Arc. Electrophysiological methods included assessment of long-term potentiation (LTP), a type of synaptic plasticity thought to underlie memory formation. Behavioral studies investigated both short-term spatial memory and associative memory. These phenomena were examined following oTau elevation. Results: Levels of phospho-CREB, histone 3 acetylation at lysine 27, and immediate early genes c-Fos and Arc, were found to be reduced after oTau elevation during memory formation. These findings led us to explore whether up-regulation of various components of the nitric oxide (NO) signaling pathway impinging onto CREB is capable of rescuing oTau-induced impairment of plasticity, memory, and CREB phosphorylation. The increase of NO levels protected against oTau-induced impairment of LTP through activation of soluble guanylyl cyclase. Similarly, the elevation of cGMP levels and stimulation of the cGMP-dependent protein kinases (PKG) re-established normal LTP after exposure to oTau. Pharmacological inhibition of cGMP degradation through inhibition of phosphodiesterase 5 (PDE5), rescued oTau-induced LTP reduction. These findings could be extrapolated to memory because PKG activation and PDE5 inhibition rescued oTau-induced memory impairment. Finally, PDE5 inhibition re-established normal elevation of CREB phosphorylation and cGMP levels after memory induction in the presence of oTau. Conclusions: Up-regulation of CREB activation through agents acting on the NO cascade might be beneficial against tau-induced synaptic and memory dysfunctions.

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KW - Soluble guanylyl cyclase

KW - Tau oligomers

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