Synaptic clusters of MHC class II molecules induced on DCs by adhesion molecule-mediated initial T-cell scanning

Hortensia De La Fuente, María Mittelbrunn, Lorena Sánchez-Martín, Miguel Vicente-Manzanares, Amalia Lamana, Ruggero Pardi, Carlos Cabañas, Francisco Sánchez-Madrid

Research output: Contribution to journalArticlepeer-review

Abstract

Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells.

Original languageEnglish
Pages (from-to)3314-3322
Number of pages9
JournalMolecular Biology of the Cell
Volume16
Issue number7
DOIs
Publication statusPublished - Jul 2005

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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