Synaptic localization of GLT-1a in the rat somatic sensory cortex

Research output: Contribution to journalArticle

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Abstract

GLT-1a, the major glutamate transporter, plays important role in both physiological and pathological conditions. Uncertainty regarding its localization in the cerebral cortex prompted us to re-examine its cellular and subcellular localization in the rat somatic sensory cortex. GLT-1a detection was sensitive to fixation; in optimal conditions ∼25% of GLT-1a+ profiles were axon terminals. GLT-1a/VGLUT1 double-labeling and pre-embedding electron microscopy studies showed that ∼50% of GLT-1a+ profiles were in the vicinity of asymmetric. synapses. Using pre-embedding electron microscopy, we found that ∼70% of GLT-1a located in the vicinity of asymmetric synapses was astracytic and ∼30% was neuronal. Post-embedding immunogold studies showed that the density of gold particles coding for GLT-1a was much higher in astrocytic processes than in axon terminals, and that in the latter they were never at the active zone. In both astrocytic processes and axon terminals most gold particles were localized in a membrane region extending for about 250 nm from active zone margin, with a peak at 140 nm for astrocytic processes and at 80 for axon terminals. We conclude that, although GLT-1a is expressed by both astrocytes and axon terminals, astrocytic GLT-1a pre-dominates at asymmetne synapses, and that the pensynaptic localization of GLT-1a in cortex is well-suited to modulate Glu concentrations at the cleft and also to restrict Glu spillover.

Original languageEnglish
Pages (from-to)108-117
Number of pages10
JournalGLIA
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

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Presynaptic Terminals
Synapses
Gold
Electron Microscopy
Amino Acid Transport System X-AG
Astrocytes
Cerebral Cortex
Uncertainty
Membranes

Keywords

  • Astrocytes
  • GLT-1/EAAT2
  • Glutamate transporters
  • Immunogold post-embedding electron microscopy
  • Neurons

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Synaptic localization of GLT-1a in the rat somatic sensory cortex. / Melone, Marcello; Bellesi, Michele; Conti, Fiorenzo.

In: GLIA, Vol. 57, No. 1, 01.01.2009, p. 108-117.

Research output: Contribution to journalArticle

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