Synaptic plasticity during recovery from permanent occlusion of the middle cerebral artery

Diego Centonze, Silvia Rossi, Anna Tortiglione, Barbara Picconi, Chiara Prosperetti, Valentina De Chiara, Giorgio Bernardi, Paolo Calabresi

Research output: Contribution to journalArticle

Abstract

Synaptic rearrangements in the peri-infarct regions are believed to contribute to the partial recovery of function that takes place after stroke. Here, we performed neurophysiological recordings from single neurons of rats with permanent occlusion of the middle cerebral artery (pMCAO) during the resolution of their neurological deficits. Our results show that complex and dynamic changes of glutamate transmission in the peri-infarct area parallel the recovery from brain infarct. We have observed that frequency and duration of spontaneous glutamate-mediated synaptic events were markedly increased in striatal neurons during the early phase of the recovery (3 days after pMCAO), due to potentiation of both NMDA (N-methyl-d-aspartate) and non-NMDA receptor-mediated transmission. In the late phase of recovery (7 days after pMCAO), glutamate transmission was still enhanced because of a selective facilitation of non-NMDA receptor-mediated transmission. Spiny projection neurons but not aspiny interneurons underwent detectable changes of synaptic excitability in the striatum following pMCAO, indicating that the process of neuronal adaptation after focal brain ischemia is cell-type-specific. Our results provide a synaptic correlate of the long-lasting brain hyperexcitability mediating recovery described with noninvasive neurophysiological approaches.

Original languageEnglish
Pages (from-to)44-53
Number of pages10
JournalNeurobiology of Disease
Volume27
Issue number1
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Neuronal Plasticity
Middle Cerebral Artery Infarction
Glutamic Acid
Neurons
Corpus Striatum
Recovery of Function
Brain
Interneurons
Brain Ischemia
Aspartic Acid
Stroke
aspartic acid receptor

Keywords

  • AMPA receptors
  • Brain ischemia
  • Electrophysiology
  • Glutamate
  • NMDA receptors
  • pMCAO
  • sEPSCs

ASJC Scopus subject areas

  • Neurology

Cite this

Synaptic plasticity during recovery from permanent occlusion of the middle cerebral artery. / Centonze, Diego; Rossi, Silvia; Tortiglione, Anna; Picconi, Barbara; Prosperetti, Chiara; De Chiara, Valentina; Bernardi, Giorgio; Calabresi, Paolo.

In: Neurobiology of Disease, Vol. 27, No. 1, 07.2007, p. 44-53.

Research output: Contribution to journalArticle

Centonze, Diego ; Rossi, Silvia ; Tortiglione, Anna ; Picconi, Barbara ; Prosperetti, Chiara ; De Chiara, Valentina ; Bernardi, Giorgio ; Calabresi, Paolo. / Synaptic plasticity during recovery from permanent occlusion of the middle cerebral artery. In: Neurobiology of Disease. 2007 ; Vol. 27, No. 1. pp. 44-53.
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