Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I

Fabio Benfenati, Flavia Valtorta, James L. Rubenstein, Fred S. Gorelick, Paul Greengard, Andrew J. Czernik

Research output: Contribution to journalArticle

Abstract

SYNAPSIN I is a synaptic vesicle-associated phosphoprotein that is involved in the modulation of neurotransmitter release1. Ca2+/calmodulin-dependent protein kinase II, which phosphorylates two sites in the carboxy-terminal region of synapsin I, causes synapsin I to dissociate from synaptic vesicles2 and increases nerotransmitter release3,4. Conversely, the dephosphorylated form of synapsin I, but not the form phosphorylated by Ca2+/calmodulin-dependent protein kinase II, inhibits neurotransmitter release4-6. The amino-terminal region of synapsin I interacts with membrane phospholipids, whereas the C-terminal region binds to a protein component of synaptic vesicles7,8. Here we demonstrate that the binding of the C-terminal region of synapsin I involves the regulatory domain of a synaptic vesicle-associated form of Ca2+/calmodulin-dependent protein kinase II. Our results indicate that this form of the kinase functions both as a binding protein for synapsin I, and as an enzyme that phosphorylates synapsin I and promotes its dissociation from the vesicles.

Original languageEnglish
Pages (from-to)417-420
Number of pages4
JournalNature
Volume359
Issue number6394
Publication statusPublished - Oct 1 1992

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    Benfenati, F., Valtorta, F., Rubenstein, J. L., Gorelick, F. S., Greengard, P., & Czernik, A. J. (1992). Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I. Nature, 359(6394), 417-420.