Abstract
Tetanus neurotoxin causes the spastic paralysis of tetanus by blocking neurotransmitter release at inhibitory synapses of the spinal cord. This is due to the penetration of the toxin inside the neuronal cytosol where it cleaves specifically VAMP/synaptobrevin, an essential component of the neuroexocytosis apparatus. Here we show that tetanus neurotoxin is internalized inside the lumen of small synaptic vesicles following the process of vesicle reuptake. Vesicle acidification is essential for the toxin translocation in the cytosol, which results in the proteolytic cleavage of VAMP/synaptobrevin and block of exocytosis.
| Original language | English |
|---|---|
| Pages (from-to) | 13310-13315 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 93 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - Nov 12 1996 |
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ASJC Scopus subject areas
- Genetics
- General
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Synaptic vesicle endocytosis mediates the entry of tetanus neurotoxin into hippocampal neurons. / Matteoli, Michela; Verderio, Claudia; Rossetto, Ornella; Iezzi, Numa; Coco, Silvia; Schiavo, Giampietro; Montecucco, Cesare.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 23, 12.11.1996, p. 13310-13315.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synaptic vesicle endocytosis mediates the entry of tetanus neurotoxin into hippocampal neurons
AU - Matteoli, Michela
AU - Verderio, Claudia
AU - Rossetto, Ornella
AU - Iezzi, Numa
AU - Coco, Silvia
AU - Schiavo, Giampietro
AU - Montecucco, Cesare
PY - 1996/11/12
Y1 - 1996/11/12
N2 - Tetanus neurotoxin causes the spastic paralysis of tetanus by blocking neurotransmitter release at inhibitory synapses of the spinal cord. This is due to the penetration of the toxin inside the neuronal cytosol where it cleaves specifically VAMP/synaptobrevin, an essential component of the neuroexocytosis apparatus. Here we show that tetanus neurotoxin is internalized inside the lumen of small synaptic vesicles following the process of vesicle reuptake. Vesicle acidification is essential for the toxin translocation in the cytosol, which results in the proteolytic cleavage of VAMP/synaptobrevin and block of exocytosis.
AB - Tetanus neurotoxin causes the spastic paralysis of tetanus by blocking neurotransmitter release at inhibitory synapses of the spinal cord. This is due to the penetration of the toxin inside the neuronal cytosol where it cleaves specifically VAMP/synaptobrevin, an essential component of the neuroexocytosis apparatus. Here we show that tetanus neurotoxin is internalized inside the lumen of small synaptic vesicles following the process of vesicle reuptake. Vesicle acidification is essential for the toxin translocation in the cytosol, which results in the proteolytic cleavage of VAMP/synaptobrevin and block of exocytosis.
UR - http://www.scopus.com/inward/record.url?scp=0029847230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029847230&partnerID=8YFLogxK
U2 - 10.1073/pnas.93.23.13310
DO - 10.1073/pnas.93.23.13310
M3 - Article
C2 - 8917587
AN - SCOPUS:0029847230
VL - 93
SP - 13310
EP - 13315
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 23
ER -