Syncoilin is required for generating maximum isometric stress in skeletal muscle but dispensable for muscle cytoarchitecture

Jianlin Zhang, Marie Louise Bang, David S. Gokhin, Yingchun Lu, Li Cui, Xiaodong Li, Yusu Gu, Nancy D. Dalton, Maria Cecilia Scimia, Kirk L. Peterson, Richard L. Lieber, Ju Chen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Syncoilin is a striated muscle-specific intermediate filament-like protein, which is part of the dystrophin-associated protein complex (DPC) at the sarcolemma and provides a link between the extracellular matrix and the cytoskeleton through its interaction with α-dystrobrevin and desmin. Its upregulation in various neuromuscular diseases suggests that syncoilin may play a role in human myopathies. To study the functional role of syncoilin in cardiac and skeletal muscle in vivo, we generated syncoilin-deficient (syncoilin -/-) mice. Our detailed analysis of these mice up to 2 yr of age revealed that syncoilin is entirely dispensable for cardiac and skeletal muscle development and maintenance of cellular structure but is required for efficient lateral force transmission during skeletal muscle contraction. Notably, syncoilin-/- skeletal muscle generates less maximal isometric stress than wild-type (WT) muscle but is as equally susceptible to eccentric contraction-induced injury as WT muscle. This suggests that syncoilin may play a supportive role for desmin in the efficient coupling of mechanical stress between the myofibril and fiber exterior. It is possible that the reduction in isometric stress production may predispose the syncoilin skeletal muscle to a dystrophic condition.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume294
Issue number5
DOIs
Publication statusPublished - May 2008

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Muscle
Skeletal Muscle
Muscles
Desmin
Dystrophin-Associated Protein Complex
Myocardium
Intermediate Filament Proteins
Sarcolemma
Neuromuscular Diseases
Mechanical Stress
Striated Muscle
Muscle Development
Myofibrils
Muscular Diseases
Cellular Structures
Muscle Contraction
Cytoskeleton
Extracellular Matrix
Up-Regulation
Maintenance

Keywords

  • Intermediate filament
  • Mutant mouse
  • Sarcomere

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Syncoilin is required for generating maximum isometric stress in skeletal muscle but dispensable for muscle cytoarchitecture. / Zhang, Jianlin; Bang, Marie Louise; Gokhin, David S.; Lu, Yingchun; Cui, Li; Li, Xiaodong; Gu, Yusu; Dalton, Nancy D.; Scimia, Maria Cecilia; Peterson, Kirk L.; Lieber, Richard L.; Chen, Ju.

In: American Journal of Physiology - Cell Physiology, Vol. 294, No. 5, 05.2008.

Research output: Contribution to journalArticle

Zhang, Jianlin ; Bang, Marie Louise ; Gokhin, David S. ; Lu, Yingchun ; Cui, Li ; Li, Xiaodong ; Gu, Yusu ; Dalton, Nancy D. ; Scimia, Maria Cecilia ; Peterson, Kirk L. ; Lieber, Richard L. ; Chen, Ju. / Syncoilin is required for generating maximum isometric stress in skeletal muscle but dispensable for muscle cytoarchitecture. In: American Journal of Physiology - Cell Physiology. 2008 ; Vol. 294, No. 5.
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