In vivo gangliosides (GS) bring about inhibitory effects on the immune response which is attributable to a weakening of the interaction between interleukine-2 (IL-2) and its receptor. In a previous paper we showed that the GS are capable of bringing about in vitro tile immunosoppressive power of cyclosporin A CyA. In this paper we have tried to associate gangliosides (GS) and low dose of CyA in the grafting of rat allogen cutis in order to have in this way in vivo a confirmation of the results previously obtained in vitro. Cutaneous strips taken from Lewis rats were grafted into Sprague Dawley rats and treated for 21 days with intraperitoneal administration of GS, of Cya, and a mixture of the two. The rats were not treated and the rats treated with GS and with CYA separately rejected the graft of tile cutis. On the other hand, the use of an association of GS and CyA brought about a successful graft in 8 rats out of 10. Splenic cells extracted 21 days after the graft from rats treated with GS and with CyA separately were stimulated in vitro with Packweed mitogene (PWM); on the contrary the cells extracted from rats treated with a combination of the two drugs did not react to the stimulation with PWM. Our results show that the GS brings about in vivo the immunosuppressive effects of low doses of CyA.
|Translated title of the contribution||Synergism between cyclosporin A and gangliosides in antirejection therapy: An experimental model in vivo|
|Number of pages||4|
|Publication status||Published - 1995|
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