Synergism Between Platelet Activating Factor and C-C Chemokines for Arachidonate Release in Human Monocytes

S. Sozzani, M. Rieppi, M. Locati, D. Zhou, F. Bussolino, P. Proost, J. Vandamme, A. Mantovani

Research output: Contribution to journalArticlepeer-review

Abstract

Monocyte Chemotactic Protein(MCP)-1 and other members of the C-C branch of the chemokine superfamily (RANTES, MIP1α/LD78, and MCP-3) induced, at chemotactic concentrations, the release of [3H]arachidonic acid in prelabeled human monocytes. Arachidonate release was potentiated (2 to 4 fold) in the presence of platelet activating factor (PAF). PAF effect was blocked by a specific receptor antagonist, WEB 2187, and was not present when inactive analogs, PAF inactive enantiomer, or lysoPAF were used. Thus, the synergistic action of PAF with C-C chemokines, in terms of arachidonate release and chemotaxis, is specific for this alkylphospholipid and is receptor mediated. Reciprocal potentiation of PAF and C-C chemokines may be important at sites of inflammation.

Original languageEnglish
Pages (from-to)761-766
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume199
Issue number2
DOIs
Publication statusPublished - Mar 15 1994

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'Synergism Between Platelet Activating Factor and C-C Chemokines for Arachidonate Release in Human Monocytes'. Together they form a unique fingerprint.

Cite this