Synovial fluid-derived T helper 17 cells correlate with inflammatory activity in arthritis, irrespectively of diagnosis

Gaetano Zizzo, Maria De Santis, Silvia Laura Bosello, Anna Laura Fedele, Giusy Peluso, Elisa Gremese, Barbara Tolusso, GianFranco Ferraccioli

Research output: Contribution to journalArticle

Abstract

We analyzed peripheral blood (PB) and synovial fluid (SF) mononuclear cells from 16 rheumatoid arthritis (RA), 9 spondyloarthritis (SpA), 3 microcrystal arthritis patients, to define the presence of Th17 and Th1 and their relationship with inflammatory activity, and TCR-zeta chain and ZAP-70 levels. Th17 were significantly higher in SF than in PB and more abundant in microcrystal arthritis patients compared to the other groups. Irrespectively of the diagnosis, SF Th17 percentages correlated with joint (SF total leukocyte count, neutrophil percentage) and systemic (C reactive protein [CRP], fibrinogen, erythrocyte sedimentation rate) inflammation markers. SF Th1 percentages directly correlated with inflammation and disease activity (CRP, swollen joint count [SJC]) indices in SpA, but not in RA patients. These observations support the role of Th17 in the pathogenesis of inflammatory arthritides. The TCR-zeta dim lymphocytes in SF were found to produce the highest amounts of cytokines including IL-17, whereas no ZAP-70 impairment was associated to Th17.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalClinical Immunology
Volume138
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • Arthritis
  • Disease activity
  • Inflammation
  • Microcrystal
  • TCR-zeta
  • Th1
  • Th17
  • ZAP-70

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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