Syntheses of deuterated leu-enkephalins and their use as internal standards for the quantification of leu-enkephalin by fast atom bombardment mass spectrometry

E. Benfenati, G. Icardi, S. Chen, R. Fanelli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have developed a synthetic method for the preparation of di- and pentadeuterated leu-enkephalin (LE), Tyr-Gly-Gly-Phe-Leu, by proton-deuterium exchange using CF3COO2H. Four to six deuterium atoms are introduced using a reaction temperature of 120°C and if 5% of 2H2O is added the di-deuterated LE is obtained. These deuterated compounds are used as internal standards to plot calibration curves of LE using fast atom bombardment mass spectrometry.

Original languageEnglish
Pages (from-to)411-419
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume28
Issue number4
DOIs
Publication statusPublished - 1990

Fingerprint

Fast Atom Bombardment Mass Spectrometry
Leucine Enkephalin
Mass spectrometry
Atoms
Deuterium
Calibration
Protons
Ion exchange
Temperature

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

@article{c2575d3a4ce04c15a631ddad57ce306e,
title = "Syntheses of deuterated leu-enkephalins and their use as internal standards for the quantification of leu-enkephalin by fast atom bombardment mass spectrometry",
abstract = "We have developed a synthetic method for the preparation of di- and pentadeuterated leu-enkephalin (LE), Tyr-Gly-Gly-Phe-Leu, by proton-deuterium exchange using CF3COO2H. Four to six deuterium atoms are introduced using a reaction temperature of 120°C and if 5{\%} of 2H2O is added the di-deuterated LE is obtained. These deuterated compounds are used as internal standards to plot calibration curves of LE using fast atom bombardment mass spectrometry.",
author = "E. Benfenati and G. Icardi and S. Chen and R. Fanelli",
year = "1990",
doi = "10.1002/jlcr.2580280407",
language = "English",
volume = "28",
pages = "411--419",
journal = "Journal of Labelled Compounds and Radiopharmaceuticals",
issn = "0362-4803",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - Syntheses of deuterated leu-enkephalins and their use as internal standards for the quantification of leu-enkephalin by fast atom bombardment mass spectrometry

AU - Benfenati, E.

AU - Icardi, G.

AU - Chen, S.

AU - Fanelli, R.

PY - 1990

Y1 - 1990

N2 - We have developed a synthetic method for the preparation of di- and pentadeuterated leu-enkephalin (LE), Tyr-Gly-Gly-Phe-Leu, by proton-deuterium exchange using CF3COO2H. Four to six deuterium atoms are introduced using a reaction temperature of 120°C and if 5% of 2H2O is added the di-deuterated LE is obtained. These deuterated compounds are used as internal standards to plot calibration curves of LE using fast atom bombardment mass spectrometry.

AB - We have developed a synthetic method for the preparation of di- and pentadeuterated leu-enkephalin (LE), Tyr-Gly-Gly-Phe-Leu, by proton-deuterium exchange using CF3COO2H. Four to six deuterium atoms are introduced using a reaction temperature of 120°C and if 5% of 2H2O is added the di-deuterated LE is obtained. These deuterated compounds are used as internal standards to plot calibration curves of LE using fast atom bombardment mass spectrometry.

UR - http://www.scopus.com/inward/record.url?scp=0025355285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025355285&partnerID=8YFLogxK

U2 - 10.1002/jlcr.2580280407

DO - 10.1002/jlcr.2580280407

M3 - Article

AN - SCOPUS:0025355285

VL - 28

SP - 411

EP - 419

JO - Journal of Labelled Compounds and Radiopharmaceuticals

JF - Journal of Labelled Compounds and Radiopharmaceuticals

SN - 0362-4803

IS - 4

ER -