Syntheses of deuterated leu-enkephalins and their use as internal standards for the quantification of leu-enkephalin by fast atom bombardment mass spectrometry

E. Benfenati, G. Icardi, S. Chen, R. Fanelli

Research output: Contribution to journalArticle

Abstract

We have developed a synthetic method for the preparation of di- and pentadeuterated leu-enkephalin (LE), Tyr-Gly-Gly-Phe-Leu, by proton-deuterium exchange using CF3COO2H. Four to six deuterium atoms are introduced using a reaction temperature of 120°C and if 5% of 2H2O is added the di-deuterated LE is obtained. These deuterated compounds are used as internal standards to plot calibration curves of LE using fast atom bombardment mass spectrometry.

Original languageEnglish
Pages (from-to)411-419
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume28
Issue number4
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

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