Synthesis and characterization of polyethylenimine-based iron oxide composites as novel contrast agents for MRI

A. Masotti, A. Pitta, G. Ortaggi, M. Corti, C. Innocenti, A. Lascialfari, M. Marinone, P. Marzola, A. Daducci, A. Sbarbati, E. Micotti, F. Orsini, G. Poletti, C. Sangregorio

Research output: Contribution to journalArticle

Abstract

Object: Use of polyethylenimines (PEIs) of different molecular weight and selected carboxylated-PEI derivatives (PEI-COOH) in the synthesis and stabilization of iron oxide nanoparticles, to obtain possible multifunctional contrast agents. Materials and methods: Oxidation of Fe(II) at slightly elevated pH and temperature resulted in the formation of highly soluble and stable nanocomposites of iron oxides and polymer. Composites were characterized and studied by atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray diffractometry, AC and DC magnetometry, NMR relaxometry and magnetic resonance imaging (MRI). Results: From AFM the dimensions of the aggregates were found to be in the ~150-250 nm size region; the mean diameter of the magnetic core of the compounds named PEI-25, PEI-500 and PEI-COOH60 resulted d ~20 ± 5 nm for PEI-25, d ~9.5 ± 1.0 nm for PEI-500 and d ~6.8 ± 1.0 nm for PEI-COOH60. In PEI-COOH60 TEM and X-ray diffractometry revealed small assemblies of mineral magnetic cores with clear indications that the main constituents are maghemite and/or magnetite as confirmed by AC and DC SQUID magnetometry. For PEI-COOH60, the study of NMR-dispersion profiles revealed r 1 and r 2 relaxivities comparable to superparamagnetic iron-oxide commercial compounds in the whole investigated frequency range 7 ≤ ν ≤ 212 MHz. Conclusion: PEI-25 was studied as possible MRI contrast agent (CA) to map the cerebral blood volume (CBV) and cerebral blood flow (CBF) in an animal model obtaining promising results. The reported compounds may be further functionalized to afford novel multifunctional systems for biomedical applications.

Original languageEnglish
Pages (from-to)77-87
Number of pages11
JournalMagnetic Resonance Materials in Physics, Biology, and Medicine
Volume22
Issue number2
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Polyethyleneimine
Contrast Media
Magnetic Resonance Imaging
Magnetometry
Atomic Force Microscopy
Transmission Electron Microscopy
ferric oxide
Cerebrovascular Circulation
X-Rays
Ferrosoferric Oxide
Nanocomposites
Nanoparticles
Minerals
Polymers

Keywords

  • Composites
  • Contrast agents
  • Iron oxide
  • MRI
  • Nanoparticles
  • Polyethylenimine

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Synthesis and characterization of polyethylenimine-based iron oxide composites as novel contrast agents for MRI. / Masotti, A.; Pitta, A.; Ortaggi, G.; Corti, M.; Innocenti, C.; Lascialfari, A.; Marinone, M.; Marzola, P.; Daducci, A.; Sbarbati, A.; Micotti, E.; Orsini, F.; Poletti, G.; Sangregorio, C.

In: Magnetic Resonance Materials in Physics, Biology, and Medicine, Vol. 22, No. 2, 04.2009, p. 77-87.

Research output: Contribution to journalArticle

Masotti, A, Pitta, A, Ortaggi, G, Corti, M, Innocenti, C, Lascialfari, A, Marinone, M, Marzola, P, Daducci, A, Sbarbati, A, Micotti, E, Orsini, F, Poletti, G & Sangregorio, C 2009, 'Synthesis and characterization of polyethylenimine-based iron oxide composites as novel contrast agents for MRI', Magnetic Resonance Materials in Physics, Biology, and Medicine, vol. 22, no. 2, pp. 77-87. https://doi.org/10.1007/s10334-008-0147-x
Masotti, A. ; Pitta, A. ; Ortaggi, G. ; Corti, M. ; Innocenti, C. ; Lascialfari, A. ; Marinone, M. ; Marzola, P. ; Daducci, A. ; Sbarbati, A. ; Micotti, E. ; Orsini, F. ; Poletti, G. ; Sangregorio, C. / Synthesis and characterization of polyethylenimine-based iron oxide composites as novel contrast agents for MRI. In: Magnetic Resonance Materials in Physics, Biology, and Medicine. 2009 ; Vol. 22, No. 2. pp. 77-87.
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abstract = "Object: Use of polyethylenimines (PEIs) of different molecular weight and selected carboxylated-PEI derivatives (PEI-COOH) in the synthesis and stabilization of iron oxide nanoparticles, to obtain possible multifunctional contrast agents. Materials and methods: Oxidation of Fe(II) at slightly elevated pH and temperature resulted in the formation of highly soluble and stable nanocomposites of iron oxides and polymer. Composites were characterized and studied by atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray diffractometry, AC and DC magnetometry, NMR relaxometry and magnetic resonance imaging (MRI). Results: From AFM the dimensions of the aggregates were found to be in the ~150-250 nm size region; the mean diameter of the magnetic core of the compounds named PEI-25, PEI-500 and PEI-COOH60 resulted d ~20 ± 5 nm for PEI-25, d ~9.5 ± 1.0 nm for PEI-500 and d ~6.8 ± 1.0 nm for PEI-COOH60. In PEI-COOH60 TEM and X-ray diffractometry revealed small assemblies of mineral magnetic cores with clear indications that the main constituents are maghemite and/or magnetite as confirmed by AC and DC SQUID magnetometry. For PEI-COOH60, the study of NMR-dispersion profiles revealed r 1 and r 2 relaxivities comparable to superparamagnetic iron-oxide commercial compounds in the whole investigated frequency range 7 ≤ ν ≤ 212 MHz. Conclusion: PEI-25 was studied as possible MRI contrast agent (CA) to map the cerebral blood volume (CBV) and cerebral blood flow (CBF) in an animal model obtaining promising results. The reported compounds may be further functionalized to afford novel multifunctional systems for biomedical applications.",
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AU - Pitta, A.

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AU - Corti, M.

AU - Innocenti, C.

AU - Lascialfari, A.

AU - Marinone, M.

AU - Marzola, P.

AU - Daducci, A.

AU - Sbarbati, A.

AU - Micotti, E.

AU - Orsini, F.

AU - Poletti, G.

AU - Sangregorio, C.

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