Synthesis and multidisciplinary characterization of polyelectrolyte multilayer-coated nanogold with improved stability toward aggregation

Subhra Mandal, Alois Bonifacio, Francesco Zanuttin, Valter Sergo, Silke Krol

Research output: Contribution to journalArticlepeer-review

Abstract

An interesting nanodrug delivery system is polyelectrolyte multilayer-coated nanogold. For better understanding of the binding of polycations or the counter-indicative deposition of polyanions on the citrate-stabilized gold nanoparticles, we used a surface-enhanced Raman spectroscopy to characterize the orientation of the polyions towards the gold surface. It was found that poly-allylamine replaces citrate molecules while the polyanion, poly-styrene sulfonate, intercalates in the citrate shell. One of the major obstacles for polyelectrolyte-coated nanogold is its tendency to agglomerate in the presence of high ion concentration as present, e.g., in blood. A novel encapsulation protocol for polyelectrolyte multilayer coating of gold nanoparticles was developed to successfully overcome this drawback. Moreover, electrostatic functionalization of the polyelectrolyte shell with a model target molecule for cancer, folic acid, induced a significant increase in the particle uptake in folate-receptor over-expressing breast cancer cell lines, VP 229 and MDA MB 231, compared to non-targeted particles or cells (non-activated macrophages) not expressing the folate receptor.

Original languageEnglish
Pages (from-to)269-280
Number of pages12
JournalColloid and Polymer Science
Volume289
Issue number3
DOIs
Publication statusPublished - Feb 2011

Keywords

  • Cancer
  • Gold nanoparticles
  • Layer-by-layer
  • Polyelectrolyte
  • SERS
  • Targeted delivery

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Polymers and Plastics
  • Materials Chemistry
  • Colloid and Surface Chemistry

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