Synthesis and use of pentadeuteroethyl ethofumesate as an internal standard for the determination of ethofumesate and its metabolites in water by gas chromatography-mass spectrometry

M. Terreni, E. Benfenati, M. Natangelo, G. Facchini, G. Pagani

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Pentadeuteroethyl ethofumesate (ETO-d5) was synthesized starting by acid hydrolysis of ethofumesate to 2,3-dihydro-2-hydroxy-3,3-dimethylbenzofuran-5-yl methanesulionate (ETO-OH) and successive ethylation with iodoethane-d5. This deuterated compound was used as an internal standard for the determination by gas chromatography-mass spectrometry in the selected-ion monitoring mode of ethofumesate (ETO) and its chief metabolites, ETO-OH and 2,3-dihydro-2-oxo-3,3-dimethylbenzofuran-5-yl methanesulfonate (ETO-K). The recovery of these substances by solid-phase extraction from drinking water was comparable to those with C18-bonded silica and Carbopack B phases. The recoveries of ETO and ETO-K were virtually complete, compared with 66% extraction for ETO-OH.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalJournal of Chromatography A
Volume688
Issue number1-2
DOIs
Publication statusPublished - Dec 30 1994

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Metabolites
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Water
ethofumesate
Recovery
Solid Phase Extraction
Silicon Dioxide
Drinking Water
Hydrolysis
Ions
Acids
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Synthesis and use of pentadeuteroethyl ethofumesate as an internal standard for the determination of ethofumesate and its metabolites in water by gas chromatography-mass spectrometry. / Terreni, M.; Benfenati, E.; Natangelo, M.; Facchini, G.; Pagani, G.

In: Journal of Chromatography A, Vol. 688, No. 1-2, 30.12.1994, p. 243-250.

Research output: Contribution to journalArticle

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