Synthesis and use of pentadeuteroethyl ethofumesate as an internal standard for the determination of ethofumesate and its metabolites in water by gas chromatography-mass spectrometry

M. Terreni, E. Benfenati, M. Natangelo, G. Facchini, G. Pagani

Research output: Contribution to journalArticle

Abstract

Pentadeuteroethyl ethofumesate (ETO-d5) was synthesized starting by acid hydrolysis of ethofumesate to 2,3-dihydro-2-hydroxy-3,3-dimethylbenzofuran-5-yl methanesulionate (ETO-OH) and successive ethylation with iodoethane-d5. This deuterated compound was used as an internal standard for the determination by gas chromatography-mass spectrometry in the selected-ion monitoring mode of ethofumesate (ETO) and its chief metabolites, ETO-OH and 2,3-dihydro-2-oxo-3,3-dimethylbenzofuran-5-yl methanesulfonate (ETO-K). The recovery of these substances by solid-phase extraction from drinking water was comparable to those with C18-bonded silica and Carbopack B phases. The recoveries of ETO and ETO-K were virtually complete, compared with 66% extraction for ETO-OH.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalJournal of Chromatography A
Volume688
Issue number1-2
DOIs
Publication statusPublished - Dec 30 1994

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Synthesis and use of pentadeuteroethyl ethofumesate as an internal standard for the determination of ethofumesate and its metabolites in water by gas chromatography-mass spectrometry'. Together they form a unique fingerprint.

  • Cite this