Synthesis, characterization and immunochemical evaluation of cephalosporin antigenic determinants

Francisco Sánchez-Sancho, Ezequiel Perez-Inestrosa, Rafael Suau, María I. Montañez, Cristobalina Mayorga, Maria J. Torres, Antonino Romano, Miguel Blanca

Research output: Contribution to journalArticlepeer-review

Abstract

Lack of knowledge of the exact chemical structure of cephalosporin antigenic determinants has hindered clinical interpretation of adverse reactions to these drugs and delayed understanding of the mechanisms involved in the specific recognition and binding of IgE molecules to these antigenic determinants. We further resolve the relationship between structure and activity of proposed antigenic chemicals, including the rational design and synthesis of these haptenic structures. Comparative RAST inhibition studies of the synthesized molecules revealed that they were recognized by IgE antibodies induced by cephalosporin antibiotics. Thus, these data indicate that recognition is mainly directed to the acyl side chain and to the β-lactam fragment that remains linked to the carrier protein in the cephalosporin conjugation course.

Original languageEnglish
Pages (from-to)148-156
Number of pages9
JournalJournal of molecular recognition : JMR
Volume16
Issue number3
DOIs
Publication statusPublished - May 2003

Keywords

  • Antibiotics
  • Cephalosporins
  • IgE
  • Immediate reactions
  • Immunochemistry
  • Lactams
  • Molecular recognition

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Computer Vision and Pattern Recognition
  • Immunology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Synthesis, characterization and immunochemical evaluation of cephalosporin antigenic determinants'. Together they form a unique fingerprint.

Cite this