Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

R. Ravazzolo; G. Bianchi-Scarra; V. Capra; P. Fiorentini; C. Garre

Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

R. Ravazzolo, G. Bianchi-Scarra, V. Capra, P. Fiorentini, C. Garre

Research output: Contribution to journal › Article › peer-review

Abstract

Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.

Original language	English
Pages (from-to)	150-153
Number of pages	4
Journal	European Journal of Haematology
Volume	44
Issue number	3
Publication status	Published - 1990

ASJC Scopus subject areas

Hematology

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abstract = "Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.",

author = "R. Ravazzolo and G. Bianchi-Scarra and V. Capra and P. Fiorentini and C. Garre",

year = "1990",

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AU - Ravazzolo, R.

AU - Bianchi-Scarra, G.

AU - Capra, V.

AU - Fiorentini, P.

AU - Garre, C.

PY - 1990

Y1 - 1990

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AB - Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.

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ER -

Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

Abstract

ASJC Scopus subject areas

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