Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

R. Ravazzolo, G. Bianchi-Scarra, V. Capra, P. Fiorentini, C. Garre

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Abstract

Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.

Original languageEnglish
Pages (from-to)150-153
Number of pages4
JournalEuropean Journal of Haematology
Volume44
Issue number3
Publication statusPublished - 1990

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ASJC Scopus subject areas

  • Hematology

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