Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

R. Ravazzolo; G. Bianchi-Scarra; V. Capra; P. Fiorentini; C. Garre

Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

R. Ravazzolo, G. Bianchi-Scarra, V. Capra, P. Fiorentini, C. Garre

Research output: Contribution to journal › Article › peer-review

Abstract

Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.

Original language	English
Pages (from-to)	150-153
Number of pages	4
Journal	European Journal of Haematology
Volume	44
Issue number	3
Publication status	Published - 1990

ASJC Scopus subject areas

Hematology

Cite this

@article{e61f2e34172f4aa8955babeb43954250,

title = "Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line",

abstract = "Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.",

author = "R. Ravazzolo and G. Bianchi-Scarra and V. Capra and P. Fiorentini and C. Garre",

year = "1990",

language = "English",

volume = "44",

pages = "150--153",

journal = "European Journal of Haematology",

issn = "0902-4441",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

}

TY - JOUR

T1 - Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

AU - Ravazzolo, R.

AU - Bianchi-Scarra, G.

AU - Capra, V.

AU - Fiorentini, P.

AU - Garre, C.

PY - 1990

Y1 - 1990

N2 - Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.

AB - Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.

UR - http://www.scopus.com/inward/record.url?scp=0025280009&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025280009&partnerID=8YFLogxK

M3 - Article

C2 - 2328786

AN - SCOPUS:0025280009

VL - 44

SP - 150

EP - 153

JO - European Journal of Haematology

JF - European Journal of Haematology

SN - 0902-4441

IS - 3

ER -

Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this