Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line

R. Ravazzolo, G. Bianchi-Scarra, V. Capra, P. Fiorentini, C. Garre

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.

Original languageEnglish
Pages (from-to)150-153
Number of pages4
JournalEuropean Journal of Haematology
Volume44
Issue number3
Publication statusPublished - 1990

Fingerprint

HL-60 Cells
Cytarabine
DNA-Binding Proteins
Nuclear Proteins
Cell Line
Proteins
Cytotoxins
Affinity Chromatography
Methionine
Leukemia
DNA
Therapeutics

ASJC Scopus subject areas

  • Hematology

Cite this

Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line. / Ravazzolo, R.; Bianchi-Scarra, G.; Capra, V.; Fiorentini, P.; Garre, C.

In: European Journal of Haematology, Vol. 44, No. 3, 1990, p. 150-153.

Research output: Contribution to journalArticle

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