Synthesis of platelet-activating factor by polymorphonuclear neutrophils stimulated with interleukin-8

Research output: Contribution to journalArticlepeer-review

Abstract

Human interleukin-8 (IL-8) was evaluated for its capability to induce the synthesis and release of platelet-activating factor (PAF) from human polymorphonuclear neutrophils (PMN). IL-8 promotes in a dose-dependent fashion (1-100 ng/ml) a rapid synthesis of PAF, which is only partially released. The synthesis of PAF is preceded by the activation of acetyl-CoA: 1-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase, suggesting that IL-8 activates the 'remodeling pathway' of PAF synthesis. By thin layer chromatography and reverse-phase high pressure liquid chromatography, we demonstrated that PAF synthesized by human PMN stimulated with IL-8 is heterogeneous: the 2-acetylated phospholipids having the biological and physicochemical characteristics of PAF include the 1-O-alkyl form, which is produced in large extent (51%), and the 1-acyl form (20%). The analysis of the individual molecular species of radyl chain indicated nine peaks, 16:0 and 18:0 being the predominant forms. These results identify PAF as a direct product of IL-8 stimulation in PMN.

Original languageEnglish
Pages (from-to)14598-14603
Number of pages6
JournalJournal of Biological Chemistry
Volume267
Issue number21
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Synthesis of platelet-activating factor by polymorphonuclear neutrophils stimulated with interleukin-8'. Together they form a unique fingerprint.

Cite this