TY - JOUR
T1 - Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics
AU - Ferrari, Raffaele
AU - Colombo, Claudio
AU - Casali, Cristina
AU - Lupi, Monica
AU - Ubezio, Paolo
AU - Falcetta, Francesca
AU - D'Incalci, Maurizio
AU - Morbidelli, Massimo
AU - Moscatelli, Davide
PY - 2013
Y1 - 2013
N2 - A delivery system based on polymer nanoparticles (NPs) is developed and tested in relevant biological conditions for breast cancer treatment. ε-Caprolactone (CL) and polyethylene glycol (PEG) copolymers have been used for the one pot synthesis of surfactant free PEGylated NPs which are monodispersed, stable in physiological conditions and have size in the range 90-250 nm. The degradation behavior of these NPs has been investigated in cell medium and a relation between degradation time and molecular weight of the starting CL-based material has been established. This allows producing NPs with controlled degradation kinetics. Finally, selected NPs have been tested in 4T1 breast cancer cells to check their toxicity and to investigate the uptake process, in order to validate their use as targeted vectors for breast cancer treatment.
AB - A delivery system based on polymer nanoparticles (NPs) is developed and tested in relevant biological conditions for breast cancer treatment. ε-Caprolactone (CL) and polyethylene glycol (PEG) copolymers have been used for the one pot synthesis of surfactant free PEGylated NPs which are monodispersed, stable in physiological conditions and have size in the range 90-250 nm. The degradation behavior of these NPs has been investigated in cell medium and a relation between degradation time and molecular weight of the starting CL-based material has been established. This allows producing NPs with controlled degradation kinetics. Finally, selected NPs have been tested in 4T1 breast cancer cells to check their toxicity and to investigate the uptake process, in order to validate their use as targeted vectors for breast cancer treatment.
KW - Degradation
KW - Emulsion polymerization
KW - Nanoparticles
KW - PEG
KW - Poly(ε-caprolactone)
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U2 - 10.1016/j.ijpharm.2013.06.020
DO - 10.1016/j.ijpharm.2013.06.020
M3 - Article
C2 - 23796832
AN - SCOPUS:84884155328
VL - 453
SP - 551
EP - 559
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 2
ER -