Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics

Raffaele Ferrari; Claudio Colombo; Cristina Casali; Monica Lupi; Paolo Ubezio; Francesca Falcetta; Maurizio D'Incalci; Massimo Morbidelli; Davide Moscatelli

doi:10.1016/j.ijpharm.2013.06.020

Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics

Raffaele Ferrari, Claudio Colombo, Cristina Casali, Monica Lupi, Paolo Ubezio, Francesca Falcetta, Maurizio D'Incalci, Massimo Morbidelli, Davide Moscatelli

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article

Abstract

A delivery system based on polymer nanoparticles (NPs) is developed and tested in relevant biological conditions for breast cancer treatment. ε-Caprolactone (CL) and polyethylene glycol (PEG) copolymers have been used for the one pot synthesis of surfactant free PEGylated NPs which are monodispersed, stable in physiological conditions and have size in the range 90-250 nm. The degradation behavior of these NPs has been investigated in cell medium and a relation between degradation time and molecular weight of the starting CL-based material has been established. This allows producing NPs with controlled degradation kinetics. Finally, selected NPs have been tested in 4T1 breast cancer cells to check their toxicity and to investigate the uptake process, in order to validate their use as targeted vectors for breast cancer treatment.

Original language	English
Pages (from-to)	551-559
Number of pages	9
Journal	International Journal of Pharmaceutics
Volume	453
Issue number	2
DOIs	https://doi.org/10.1016/j.ijpharm.2013.06.020
Publication status	Published - 2013

Keywords

Degradation
Emulsion polymerization
Nanoparticles
PEG
Poly(ε-caprolactone)

ASJC Scopus subject areas

Pharmaceutical Science

Access to Document

10.1016/j.ijpharm.2013.06.020

Fingerprint Dive into the research topics of 'Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics'. Together they form a unique fingerprint.

Cite this

Ferrari, R., Colombo, C., Casali, C., Lupi, M., Ubezio, P., Falcetta, F., D'Incalci, M., Morbidelli, M., & Moscatelli, D. (2013). Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics. International Journal of Pharmaceutics, 453(2), 551-559. https://doi.org/10.1016/j.ijpharm.2013.06.020

Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics. / Ferrari, Raffaele; Colombo, Claudio; Casali, Cristina; Lupi, Monica ; Ubezio, Paolo; Falcetta, Francesca; D'Incalci, Maurizio; Morbidelli, Massimo; Moscatelli, Davide.

In: International Journal of Pharmaceutics, Vol. 453, No. 2, 2013, p. 551-559.

Research output: Contribution to journal › Article

@article{4594c925fab24d71b7a721eb8cc43ac0,

title = "Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics",

abstract = "A delivery system based on polymer nanoparticles (NPs) is developed and tested in relevant biological conditions for breast cancer treatment. ε-Caprolactone (CL) and polyethylene glycol (PEG) copolymers have been used for the one pot synthesis of surfactant free PEGylated NPs which are monodispersed, stable in physiological conditions and have size in the range 90-250 nm. The degradation behavior of these NPs has been investigated in cell medium and a relation between degradation time and molecular weight of the starting CL-based material has been established. This allows producing NPs with controlled degradation kinetics. Finally, selected NPs have been tested in 4T1 breast cancer cells to check their toxicity and to investigate the uptake process, in order to validate their use as targeted vectors for breast cancer treatment.",

keywords = "Degradation, Emulsion polymerization, Nanoparticles, PEG, Poly(ε-caprolactone)",

author = "Raffaele Ferrari and Claudio Colombo and Cristina Casali and Monica Lupi and Paolo Ubezio and Francesca Falcetta and Maurizio D'Incalci and Massimo Morbidelli and Davide Moscatelli",

year = "2013",

doi = "10.1016/j.ijpharm.2013.06.020",

language = "English",

volume = "453",

pages = "551--559",

journal = "International Journal of Pharmaceutics",

issn = "0378-5173",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Synthesis of surfactant free PCL-PEG brushed nanoparticles with tunable degradation kinetics

AU - Ferrari, Raffaele

AU - Colombo, Claudio

AU - Casali, Cristina

AU - Lupi, Monica

AU - Ubezio, Paolo

AU - Falcetta, Francesca

AU - D'Incalci, Maurizio

AU - Morbidelli, Massimo

AU - Moscatelli, Davide

PY - 2013

Y1 - 2013

N2 - A delivery system based on polymer nanoparticles (NPs) is developed and tested in relevant biological conditions for breast cancer treatment. ε-Caprolactone (CL) and polyethylene glycol (PEG) copolymers have been used for the one pot synthesis of surfactant free PEGylated NPs which are monodispersed, stable in physiological conditions and have size in the range 90-250 nm. The degradation behavior of these NPs has been investigated in cell medium and a relation between degradation time and molecular weight of the starting CL-based material has been established. This allows producing NPs with controlled degradation kinetics. Finally, selected NPs have been tested in 4T1 breast cancer cells to check their toxicity and to investigate the uptake process, in order to validate their use as targeted vectors for breast cancer treatment.

AB - A delivery system based on polymer nanoparticles (NPs) is developed and tested in relevant biological conditions for breast cancer treatment. ε-Caprolactone (CL) and polyethylene glycol (PEG) copolymers have been used for the one pot synthesis of surfactant free PEGylated NPs which are monodispersed, stable in physiological conditions and have size in the range 90-250 nm. The degradation behavior of these NPs has been investigated in cell medium and a relation between degradation time and molecular weight of the starting CL-based material has been established. This allows producing NPs with controlled degradation kinetics. Finally, selected NPs have been tested in 4T1 breast cancer cells to check their toxicity and to investigate the uptake process, in order to validate their use as targeted vectors for breast cancer treatment.

KW - Degradation

KW - Emulsion polymerization

KW - Nanoparticles

KW - PEG

KW - Poly(ε-caprolactone)

UR - http://www.scopus.com/inward/record.url?scp=84884155328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884155328&partnerID=8YFLogxK

U2 - 10.1016/j.ijpharm.2013.06.020

DO - 10.1016/j.ijpharm.2013.06.020

M3 - Article

C2 - 23796832

AN - SCOPUS:84884155328

VL - 453

SP - 551

EP - 559

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 2

ER -