Abstract
| Original language | English |
|---|---|
| Article number | 207 |
| Journal | International Journal of Molecular Sciences |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2018 |
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Synthesized Heparan Sulfate Competitors Attenuate Pseudomonas aeruginosa Lung Infection. / Lorè, NI; Veraldi, N; Riva, C; Sipione, B; Spagnuolo, Lorenza; De Fino, I; Melessike, M; Calzi, E; Bragonzi, A; Naggi, A; Cigana, C.
In: International Journal of Molecular Sciences, Vol. 19, No. 1, 207, 2018.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Synthesized Heparan Sulfate Competitors Attenuate Pseudomonas aeruginosa Lung Infection
AU - Lorè, NI
AU - Veraldi, N
AU - Riva, C
AU - Sipione, B
AU - Spagnuolo, Lorenza
AU - De Fino, I
AU - Melessike, M
AU - Calzi, E
AU - Bragonzi, A
AU - Naggi, A
AU - Cigana, C
PY - 2018
Y1 - 2018
N2 - Several chronic respiratory diseases are characterized by recurrent and/or persistent infections, chronic inflammatory responses and tissue remodeling, including increased levels of glycosaminoglycans which are known structural components of the airways. Among glycosaminoglycans, heparan sulfate (HS) has been suggested to contribute to excessive inflammatory responses. Here, we aim at (i) investigating whether long-term infection by Pseudomonas aeruginosa, one of the most worrisome threat in chronic respiratory diseases, may impact HS levels, and (ii) exploring HS competitors as potential anti-inflammatory drugs during P. aeruginosa pneumonia. P. aeruginosa clinical strains and ad-hoc synthesized HS competitors were used in vitro and in murine models of lung infection. During long-term chronic P. aeruginosa colonization, infected mice showed higher heparin/HS levels, evaluated by high performance liquid chromatography-mass spectrometry after selective enzymatic digestion, compared to uninfected mice. Among HS competitors, an N-acetyl heparin and a glycol-split heparin dampened leukocyte recruitment and cytokine/chemokine production induced by acute and chronic P. aeruginosa pneumonia in mice. Furthermore, treatment with HS competitors reduced bacterial burden during chronic murine lung infection. In vitro, P. aeruginosa biofilm formation decreased upon treatment with HS competitors. Overall, these findings support further evaluation of HS competitors as a novel therapy to counteract inflammation and infection during P. aeruginosa pneumonia.
AB - Several chronic respiratory diseases are characterized by recurrent and/or persistent infections, chronic inflammatory responses and tissue remodeling, including increased levels of glycosaminoglycans which are known structural components of the airways. Among glycosaminoglycans, heparan sulfate (HS) has been suggested to contribute to excessive inflammatory responses. Here, we aim at (i) investigating whether long-term infection by Pseudomonas aeruginosa, one of the most worrisome threat in chronic respiratory diseases, may impact HS levels, and (ii) exploring HS competitors as potential anti-inflammatory drugs during P. aeruginosa pneumonia. P. aeruginosa clinical strains and ad-hoc synthesized HS competitors were used in vitro and in murine models of lung infection. During long-term chronic P. aeruginosa colonization, infected mice showed higher heparin/HS levels, evaluated by high performance liquid chromatography-mass spectrometry after selective enzymatic digestion, compared to uninfected mice. Among HS competitors, an N-acetyl heparin and a glycol-split heparin dampened leukocyte recruitment and cytokine/chemokine production induced by acute and chronic P. aeruginosa pneumonia in mice. Furthermore, treatment with HS competitors reduced bacterial burden during chronic murine lung infection. In vitro, P. aeruginosa biofilm formation decreased upon treatment with HS competitors. Overall, these findings support further evaluation of HS competitors as a novel therapy to counteract inflammation and infection during P. aeruginosa pneumonia.
U2 - 10.3390/ijms19010207
DO - 10.3390/ijms19010207
M3 - Article
VL - 19
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 1
M1 - 207
ER -