Systematic review: Allogenic use of stromal vascular fraction (SVF) and decellularized extracellular matrices (ECM) as advanced therapy medicinal products (ATMP) in tissue regeneration

Pietro Gentile, Aris Sterodimas, Jacopo Pizzicannella, Laura Dionisi, Domenico De Fazio, Claudio Calabrese, Simone Garcovich

Research output: Contribution to journalReview articlepeer-review


Stromal vascular fraction (SVF) containing adipose stem cells (ASCs) has been used for many years in regenerative plastic surgery for autologous applications, without any focus on their potential allogenic role. Allogenic SVF transplants could be based on the possibility to use decellularized extracellular matrix (ECM) as a scaffold from a donor then re‐cellularized by ASCs of the recipient, in order to develop the advanced therapy medicinal products (ATMP) in fully personalized clinical approaches. A systematic review of this field has been realized in accordance with the Preferred Reporting for Items for Systematic Reviews and Meta‐Analyses‐Protocols (PRISMA‐P) guidelines. Multistep research of the PubMed, Embase, MEDLINE, Pre‐MEDLINE, PsycINFO, CINAHL,, Scopus database, and Cochrane databases has been conducted to identify articles and investigations on human allogenic ASCs transplant for clinical use. Of the 341 articles identified, 313 were initially assessed for eligibility on the basis of the abstract. Of these, only 29 met all the predetermined criteria for inclusion according to the PICOS (patients, intervention, comparator, outcomes, and study design) approach, and 19 have been included in quantitative synthesis (meta‐analysis). Ninety‐one percent of the studies previously screened (284 papers) were focused on the in vitro results and pre‐clinical experiments. The allogenic use regarded the treatment of perianal fistulas, diabetic foot ulcers, knee osteoarthritis, acute respiratory distress syndrome, refractory rheumatoid arthritis, pediatrics disease, fecal incontinence, ischemic heart disease, autoimmune encephalomyelitis, lateral epicondylitis, and soft tissue defects. The information analyzed suggested the safety and efficacy of allogenic ASCs and ECM transplants without major side effects.

Original languageEnglish
Article number4982
Pages (from-to)1-14
Number of pages14
JournalInternational Journal of Molecular Sciences
Issue number14
Publication statusPublished - Jul 2 2020


  • Adipose stem cells
  • Allogenic ASCs
  • Allogenic fat graft
  • Allogenic fat transplants
  • Allogenic SVF
  • ASCs
  • Fat graft
  • Stromal vascular fraction
  • SVF

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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