Systemic genotoxic effects produced by light, and synergism with cigarette smoke in the respiratory tract of hairless mice

Roumen M. Balansky, Alberto Izzotti, Francesco D'Agostini, Anna Camoirano, Maria Bagnasco, Ronald A. Lubet, Silvio De Flora

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

No information is available on the interaction between cigarette smoke, the most important man-made carcinogen, and light, the most widespread natural carcinogen. In order to clarify this issue, SKH-1 hairless mice were exposed to environmental smoke and/or to the light emitted by sunlight-simulating halogen quartz bulbs. After 28 days, intermediate biomarkers were evaluated in skin, respiratory tract, bone marrow and peripheral blood. The results showed that, individually, the light produced extensive alterations not only in the skin but even at a systemic level, as shown by formation of bulky DNA adducts in both lung and bone marrow and induction of cytogenetic damage in bone marrow and peripheral blood erythrocytes. Smoke damaged the respiratory tract and produced significant alterations in the skin as well as an evident cytogenetic damage in both bone marrow and peripheral blood. Interestingly, as compared with exposure to smoke only, alternate daily cycles of exposure to both light and smoke significantly increased malondialdehyde concentrations and DNA adduct levels in lung and the frequency of micronuclei in pulmonary alveolar macrophages. The oral administration of sulindac, a non-steroidal anti-inflammatory drug, attenuated several biomarker alterations due to the combined exposure of mice to light and smoke. In conclusion, the light induces a systemic genotoxic damage, which is presumably due to the UV-mediated formation in the skin of long-lived derivatives, such as aldehydes. This damage may mechanistically be involved in light-related hematopoietic malignancies. In addition, the light displayed an insofar unsuspected synergism with smoke in the induction of DNA damage in the respiratory tract.

Original languageEnglish
Pages (from-to)1525-1532
Number of pages8
JournalCarcinogenesis
Volume24
Issue number9
DOIs
Publication statusPublished - Sep 1 2003

Fingerprint

Hairless Mouse
Smoke
Tobacco Products
Respiratory System
Light
Bone Marrow
Skin
DNA Adducts
Alveolar Macrophages
Cytogenetics
Carcinogens
Biomarkers
Sulindac
Lung
Quartz
Halogens
Sunlight
Hematologic Neoplasms
Malondialdehyde
Aldehydes

ASJC Scopus subject areas

  • Cancer Research

Cite this

Systemic genotoxic effects produced by light, and synergism with cigarette smoke in the respiratory tract of hairless mice. / Balansky, Roumen M.; Izzotti, Alberto; D'Agostini, Francesco; Camoirano, Anna; Bagnasco, Maria; Lubet, Ronald A.; De Flora, Silvio.

In: Carcinogenesis, Vol. 24, No. 9, 01.09.2003, p. 1525-1532.

Research output: Contribution to journalArticle

Balansky, Roumen M. ; Izzotti, Alberto ; D'Agostini, Francesco ; Camoirano, Anna ; Bagnasco, Maria ; Lubet, Ronald A. ; De Flora, Silvio. / Systemic genotoxic effects produced by light, and synergism with cigarette smoke in the respiratory tract of hairless mice. In: Carcinogenesis. 2003 ; Vol. 24, No. 9. pp. 1525-1532.
@article{d8b612d4a7fd4d5188ddd6828a844e58,
title = "Systemic genotoxic effects produced by light, and synergism with cigarette smoke in the respiratory tract of hairless mice",
abstract = "No information is available on the interaction between cigarette smoke, the most important man-made carcinogen, and light, the most widespread natural carcinogen. In order to clarify this issue, SKH-1 hairless mice were exposed to environmental smoke and/or to the light emitted by sunlight-simulating halogen quartz bulbs. After 28 days, intermediate biomarkers were evaluated in skin, respiratory tract, bone marrow and peripheral blood. The results showed that, individually, the light produced extensive alterations not only in the skin but even at a systemic level, as shown by formation of bulky DNA adducts in both lung and bone marrow and induction of cytogenetic damage in bone marrow and peripheral blood erythrocytes. Smoke damaged the respiratory tract and produced significant alterations in the skin as well as an evident cytogenetic damage in both bone marrow and peripheral blood. Interestingly, as compared with exposure to smoke only, alternate daily cycles of exposure to both light and smoke significantly increased malondialdehyde concentrations and DNA adduct levels in lung and the frequency of micronuclei in pulmonary alveolar macrophages. The oral administration of sulindac, a non-steroidal anti-inflammatory drug, attenuated several biomarker alterations due to the combined exposure of mice to light and smoke. In conclusion, the light induces a systemic genotoxic damage, which is presumably due to the UV-mediated formation in the skin of long-lived derivatives, such as aldehydes. This damage may mechanistically be involved in light-related hematopoietic malignancies. In addition, the light displayed an insofar unsuspected synergism with smoke in the induction of DNA damage in the respiratory tract.",
author = "Balansky, {Roumen M.} and Alberto Izzotti and Francesco D'Agostini and Anna Camoirano and Maria Bagnasco and Lubet, {Ronald A.} and {De Flora}, Silvio",
year = "2003",
month = "9",
day = "1",
doi = "10.1093/carcin/bgg108",
language = "English",
volume = "24",
pages = "1525--1532",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Systemic genotoxic effects produced by light, and synergism with cigarette smoke in the respiratory tract of hairless mice

AU - Balansky, Roumen M.

AU - Izzotti, Alberto

AU - D'Agostini, Francesco

AU - Camoirano, Anna

AU - Bagnasco, Maria

AU - Lubet, Ronald A.

AU - De Flora, Silvio

PY - 2003/9/1

Y1 - 2003/9/1

N2 - No information is available on the interaction between cigarette smoke, the most important man-made carcinogen, and light, the most widespread natural carcinogen. In order to clarify this issue, SKH-1 hairless mice were exposed to environmental smoke and/or to the light emitted by sunlight-simulating halogen quartz bulbs. After 28 days, intermediate biomarkers were evaluated in skin, respiratory tract, bone marrow and peripheral blood. The results showed that, individually, the light produced extensive alterations not only in the skin but even at a systemic level, as shown by formation of bulky DNA adducts in both lung and bone marrow and induction of cytogenetic damage in bone marrow and peripheral blood erythrocytes. Smoke damaged the respiratory tract and produced significant alterations in the skin as well as an evident cytogenetic damage in both bone marrow and peripheral blood. Interestingly, as compared with exposure to smoke only, alternate daily cycles of exposure to both light and smoke significantly increased malondialdehyde concentrations and DNA adduct levels in lung and the frequency of micronuclei in pulmonary alveolar macrophages. The oral administration of sulindac, a non-steroidal anti-inflammatory drug, attenuated several biomarker alterations due to the combined exposure of mice to light and smoke. In conclusion, the light induces a systemic genotoxic damage, which is presumably due to the UV-mediated formation in the skin of long-lived derivatives, such as aldehydes. This damage may mechanistically be involved in light-related hematopoietic malignancies. In addition, the light displayed an insofar unsuspected synergism with smoke in the induction of DNA damage in the respiratory tract.

AB - No information is available on the interaction between cigarette smoke, the most important man-made carcinogen, and light, the most widespread natural carcinogen. In order to clarify this issue, SKH-1 hairless mice were exposed to environmental smoke and/or to the light emitted by sunlight-simulating halogen quartz bulbs. After 28 days, intermediate biomarkers were evaluated in skin, respiratory tract, bone marrow and peripheral blood. The results showed that, individually, the light produced extensive alterations not only in the skin but even at a systemic level, as shown by formation of bulky DNA adducts in both lung and bone marrow and induction of cytogenetic damage in bone marrow and peripheral blood erythrocytes. Smoke damaged the respiratory tract and produced significant alterations in the skin as well as an evident cytogenetic damage in both bone marrow and peripheral blood. Interestingly, as compared with exposure to smoke only, alternate daily cycles of exposure to both light and smoke significantly increased malondialdehyde concentrations and DNA adduct levels in lung and the frequency of micronuclei in pulmonary alveolar macrophages. The oral administration of sulindac, a non-steroidal anti-inflammatory drug, attenuated several biomarker alterations due to the combined exposure of mice to light and smoke. In conclusion, the light induces a systemic genotoxic damage, which is presumably due to the UV-mediated formation in the skin of long-lived derivatives, such as aldehydes. This damage may mechanistically be involved in light-related hematopoietic malignancies. In addition, the light displayed an insofar unsuspected synergism with smoke in the induction of DNA damage in the respiratory tract.

UR - http://www.scopus.com/inward/record.url?scp=0141590664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141590664&partnerID=8YFLogxK

U2 - 10.1093/carcin/bgg108

DO - 10.1093/carcin/bgg108

M3 - Article

VL - 24

SP - 1525

EP - 1532

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 9

ER -