T and B cell responses to cytomegalovirus antigens in healthy blood donors and bone marrow transplant recipients

Francesco Lolli, Vivi Anne Sundqvist, Antonella Castagna, Per Ljungman, Annika Linde, Gudrun Andersson, Tomas Olsson, Britta Wahren

Research output: Contribution to journalArticlepeer-review

Abstract

We measured the production of interferon-gamma (IFN-γ) from single T cells and the T cell proliferative response to different cytomegalovirus (CMV) antigens in healthy blood donors and bone marrow transplant recipients. The antigens consisted of a CMV nuclear antigen (CMV na) containing the pp65-kDa matrix protein and the immediate early antigens but lacking CMV glycoproteins, and an antigen comprising native CMV glycoproteins (CMV gp). We also measured the IgG antibodies to CMV na and CMV gp. The T cells reacted to CMV na in CMV seropositive blood donors both with the production of IFN-γ and with proliferation, while bone marrow transplant recipients had a deficient T cell response. After stimulation with CMV gp, no T cell response could be observed in CMV seropositive subjects. IgG antibodies to CMV na coexisted in plasma with similar levels of antibodies to CMV gp.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume7
Issue number1
DOIs
Publication statusPublished - 1993

Keywords

  • B cell
  • Bone marrow transplantation
  • Cytomegalovirus
  • Interferon type II
  • T cell

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

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