T-cell clones in human trichinellosis: Evidence for a mixed Th1/Th2 response

C. Della Bella, M. Benagiano, M. De Gennaro, M. A. Gomez-Morales, A. Ludovisi, S. D'Elios, S. Luchi, E. Pozio, M. M. D'Elios, F. Bruschi

Research output: Contribution to journalArticlepeer-review


In humans, studies on the cellular immune response against Trichinella are scarce. Aim of this study was to characterize the cytokine profile of T cells specific for Trichinella britovi in trichinellosis patients. Peripheral blood mononuclear cells (PBMC) were obtained from five patients involved in a trichinellosis outbreak caused by T. britovi, which occurred in 2013 in Tuscany (Italy). All the patients resulted positive for Trichinella-specific IgG, IgE and presented eosinophilia. T cells were investigated for their proliferation to excretory/secretory antigens from Trichinella spiralis muscle larvae (TsES) and for their cytokine profile. A total of 284 CD4+ and 42 CD8+ T-cell clones were obtained from the TsES-specific T-cell lines from PBMC. All T-cell clones proliferated in response to mitogen. Of the 284 CD4+ T-cell clones generated from TsES-specific T-cell lines, 135 (47%) proliferated significantly to TsES; 26% CD8+ T-cell clones showed proliferation to TsES. In the series of the 135 TsES-specific CD4+ clones, 51% expressed a Th2 profile, 30% a Th0 and 19% Th1. In the series of the 11 TsES-specific CD8+ T-cell clones, 18% were Tc2, 45% Tc0 and 36% Tc1. In human trichinellosis, the cellular immune response is, during the chronic phase, mixed Th1/Th2.

Original languageEnglish
Article numbere12412
JournalParasite Immunology
Issue number3
Publication statusPublished - Mar 1 2017


  • eosinophilia
  • human trichinellosis
  • IgE
  • T-cell clones
  • Th1/Th2 mixed response

ASJC Scopus subject areas

  • Parasitology
  • Immunology


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