T-cell proliferation to subinfectious SIV correlates with lack of infection after challenge of macaques

Mario Clerici, Edward A. Clark, Patricia Polacino, Inger Axberg, LaRene Kuller, Naomi I. Casey, William R. Morton, Gene M. Shearer, Raoul E. Benveniste

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To analyze correlates of protection in macaques exposed to SIV. Methods: Peripheral blood mononuclear cells (PBMC) from macaques inoculated intrarectally with various dilutions of SIV were examined for their in vitro proliferative response to SIV envelope peptides and generation of SIV-specific antibodies. Some macaques previously exposed intravenously to subinfectious doses of SIV were subsequently challenged 16 months later with an infectious intrarectal dose of SIV. Results: The viral-specific immune responses of macaques exposed to infectious doses of SIV were characterized by generation of antibodies and weak or undetectable T-cell-mediated responses. In contrast, macaques inoculated with doses of SIV below the threshold required for seroconversion and recovery of virus exhibited T-cell proliferation in response to SIV envelope synthetic peptides. The macaques that had previously been exposed to SIV resisted the subsequent virus challenge, whereas the naive macaques (never exposed to SIV) all became infected. Conclusions: The inability to productively infect macaques previously exposed to subinfectious doses of SIV suggests that a T-cell-mediated response may confer long-term protection against infection, and that AIDS vaccines should be designed to optimize the cellular arm of the immune response.

Original languageEnglish
Pages (from-to)1391-1395
Number of pages5
JournalAIDS (London, England)
Volume8
Issue number10
Publication statusPublished - Oct 1994

Keywords

  • AIDS vaccines
  • Cell-mediated immune response
  • Intrarectal virus inoculation
  • Mucosal infection
  • SIV
  • Synthetic peptides
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'T-cell proliferation to subinfectious SIV correlates with lack of infection after challenge of macaques'. Together they form a unique fingerprint.

Cite this