T cell receptor beta chain from sea bream (Sparus aurata): Molecular cloning, expression and modelling of the complexes with MHC class I

Elisa Randelli, Viviana Scala, Daniela Casani, Susan Costantini, Angelo Facchiano, Massimo Mazzini, Giuseppe Scapigliati, Francesco Buonocore

Research output: Contribution to journalArticle

Abstract

The T cell receptor is a fundamental mediator of the adaptive immune responses, since TR αβ on T cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (MHC) on APC cells. In the present study, we report the cloning of six TRB chains cDNA sequences from gilthead sea bream (Sparus aurata), a fish of high economical impact in South Mediterranean aquaculture. The V-BETA domains have the canonical features of known teleost and mammalian TR V-BETA domains and have been divided in four different subgroups. A multiple alignment of the six sea bream TRB chains with other known TRB sequences was assembled and showed the conservation of the four cysteine residues involved in disulphide bonds and of some amino acids with an important role in the assembly and signalling of the TR αβ/CD3 complex. Real-time PCR analysis was used to investigate TRB basal expression, that was maximum in the thymus followed by gut, and TRB in vitro expression after stimulation with LPS or PHA-L at 4 and 24 h (only the 4 h stimulation with LPS gave a significant effect). Moreover, the 3D structures of sea bream TRB chains and MHC-I were predicted by homology modelling with the final aim to investigate the interaction surface in the V-BETA/MHC-I complexes.

Original languageEnglish
Pages (from-to)2017-2027
Number of pages11
JournalMolecular Immunology
Volume45
Issue number7
DOIs
Publication statusPublished - Apr 2008

Keywords

  • 3D structure
  • MHC class I
  • Real time PCR
  • Sparus aurata
  • T cell antigen receptor
  • TRB

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

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  • Cite this

    Randelli, E., Scala, V., Casani, D., Costantini, S., Facchiano, A., Mazzini, M., Scapigliati, G., & Buonocore, F. (2008). T cell receptor beta chain from sea bream (Sparus aurata): Molecular cloning, expression and modelling of the complexes with MHC class I. Molecular Immunology, 45(7), 2017-2027. https://doi.org/10.1016/j.molimm.2007.10.027