Abstract
Ig gene rearrangements represent markers of lineage, clonality, and differentiation of B cells, allowing a molecular diagnosis and immunogenotypic classification of B-cell neoplasms. We sought to apply a similar approach to the study of T-cell populations by analyzing rearrangements of the T-cell receptor β-chain (T(β)) gene. Our analysis, by Southern blotting hybridization using T(β)-specific probes of DNAs from polyclonal T cells and from 12 T-cell tumors, indicates that T(β) gene rearrangement patterns can be used as markers of (i) lineage, allowing the identification of polyclonal T-cell populations, and (ii) clonality, allowing the detection of monoclonal T-cell tumors. In addition, our data indicate that T(β) gene rearrangements represent early and general markers of T-cell differentiation since they are detectable in histologically different tumors at all stages of T-cell development. The ability to determine lineage, clonality, and stage of differentiation has significant implications for future experimental and clinical studies on normal and neoplastic T cells.
| Original language | English |
|---|---|
| Pages (from-to) | 3460-3464 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 82 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 1985 |
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ASJC Scopus subject areas
- Genetics
- General
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T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms. / Flug, F.; Pelicci, P. G.; Bonetti, F.; Knowles, D. M.; Dalla-Favera, R.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 10, 1985, p. 3460-3464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms
AU - Flug, F.
AU - Pelicci, P. G.
AU - Bonetti, F.
AU - Knowles, D. M.
AU - Dalla-Favera, R.
PY - 1985
Y1 - 1985
N2 - Ig gene rearrangements represent markers of lineage, clonality, and differentiation of B cells, allowing a molecular diagnosis and immunogenotypic classification of B-cell neoplasms. We sought to apply a similar approach to the study of T-cell populations by analyzing rearrangements of the T-cell receptor β-chain (T(β)) gene. Our analysis, by Southern blotting hybridization using T(β)-specific probes of DNAs from polyclonal T cells and from 12 T-cell tumors, indicates that T(β) gene rearrangement patterns can be used as markers of (i) lineage, allowing the identification of polyclonal T-cell populations, and (ii) clonality, allowing the detection of monoclonal T-cell tumors. In addition, our data indicate that T(β) gene rearrangements represent early and general markers of T-cell differentiation since they are detectable in histologically different tumors at all stages of T-cell development. The ability to determine lineage, clonality, and stage of differentiation has significant implications for future experimental and clinical studies on normal and neoplastic T cells.
AB - Ig gene rearrangements represent markers of lineage, clonality, and differentiation of B cells, allowing a molecular diagnosis and immunogenotypic classification of B-cell neoplasms. We sought to apply a similar approach to the study of T-cell populations by analyzing rearrangements of the T-cell receptor β-chain (T(β)) gene. Our analysis, by Southern blotting hybridization using T(β)-specific probes of DNAs from polyclonal T cells and from 12 T-cell tumors, indicates that T(β) gene rearrangement patterns can be used as markers of (i) lineage, allowing the identification of polyclonal T-cell populations, and (ii) clonality, allowing the detection of monoclonal T-cell tumors. In addition, our data indicate that T(β) gene rearrangements represent early and general markers of T-cell differentiation since they are detectable in histologically different tumors at all stages of T-cell development. The ability to determine lineage, clonality, and stage of differentiation has significant implications for future experimental and clinical studies on normal and neoplastic T cells.
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U2 - 10.1073/pnas.82.10.3460
DO - 10.1073/pnas.82.10.3460
M3 - Article
C2 - 2987928
AN - SCOPUS:3342989105
VL - 82
SP - 3460
EP - 3464
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 10
ER -