T cell receptor heterogeneity in γδ T cell clones from intestinal biopsies of patients with celiac disease

Gennaro De Libero, Maria Paola Rocci, Giulia Casorati, Claudia Giachino, Giuseppina Oderda, Kaveth Tavassoli, Nicola Migone

Research output: Contribution to journalArticlepeer-review

Abstract

In celiac disease large numbers of γδ T lymphocytes infiltrate the intestinal epithelia. We have isolated intestinal γδ T cell clones from patients with celiac disease and have analyzed their T cell receptor repertoire. T cell lines and clones were obtained from jejunal biopsies of 14 celiac patients and 12 individuals without celiac disease. These were analyzed by staining with monoclonal antibodies against CD3, αβ and γδ T cell receptor, by Southern blot with γ- and δ-specific probes and by polymerase chain reaction using Vδ-specific oligonucleotides. Intestinal γδ cells from patients with celiac disease differed from those of controls with normal jejunal histology in that Vδ1+ cells and Vδ1-Vδ2- cells were significantly increased. There was no evidence of the expansion of one or more clones expressing particular types of γδ T cell receptor.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalEuropean Journal of Immunology
Volume23
Issue number2
Publication statusPublished - Feb 1993

Keywords

  • Celiac disease
  • Intraepithelial lymphocytes
  • T cell clones
  • T cell receptor γδ
  • Vδ genes

ASJC Scopus subject areas

  • Immunology

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