TY - JOUR
T1 - T cell receptor repertoire in B cell lymphoproliferative lesions in primary Sjogren's syndrome
AU - Pivetta, Barbara
AU - De Vita, Salvatore
AU - Ferraccioli, Gianfranco
AU - De Re, Valli
AU - Gloghini, Annunziata
AU - Marzotto, Alessandra
AU - Caruso, Giuseppe
AU - Dolcetti, Riccardo
AU - Bartoli, Ettore
AU - Carbone, Antonino
AU - Boiocchi, Mauro
PY - 1999
Y1 - 1999
N2 - Objective. Studies have analyzed T cell receptor (TCR)-Vβ in benign, minor salivary or lacrimal gland, or kidney lesions in Sjogren's syndrome (SS). We investigated SS related lymphoproliferative lesions. Methods. By 'family' reverse transcriptase polymerase chain reaction, we studied the expression of 20 different TCR-Vβ families in parotid lymphoproliferative lesions and peripheral blood lymphocytes (PBL) from 7 patients with primary SS, in PBL from 6 primary SS patients with no associated lymphoproliferative disorder, and in activated PBL from 2 healthy controls. T cell clonal expansion was investigated in 10 Vβ families (i.e., the most expanded ones and those previously implicated in SS pathogenesis) by single strand conformation polymorphism (SSCP) analysis. Frozen sections from parotid gland specimens were tested by immunohistochemistry for the expansion of selected Vβ families. Viral infection within the parotid lesions and serum autoantibody response were also studied. Results. An unrestricted Vβ pattern was observed. The most widely expressed Vβ family in parotid lesions was Vβ2, and Vβ immunohistochemistry results were concordant with Vβ mRNA findings. A similar pattern was observed in PBL, although the Vβ2 family was expressed at lower levels. The parotid/PBL ratio was occasionally > 1.8-2.0 (indicative of local Vβ overexpression) in different Vβ families. T cell expansion proved to be largely polyclonal by SSCP analysis, and scattered T cell clonotypes were detected within different Vβ families, with a different pattern from patient to patient. Conclusion. Our observations in SS related lymphoproliferative lesions largely reflect previous evidence in fully benign lesions. The pathogenetic events involved in autoimmune benign lesions in SS may then persist and play a role in SS related lymphoproliferative disorders. The link between the observed TCR-Vβ repertoire and specific local triggering (auto)antigens remains to be elucidated.
AB - Objective. Studies have analyzed T cell receptor (TCR)-Vβ in benign, minor salivary or lacrimal gland, or kidney lesions in Sjogren's syndrome (SS). We investigated SS related lymphoproliferative lesions. Methods. By 'family' reverse transcriptase polymerase chain reaction, we studied the expression of 20 different TCR-Vβ families in parotid lymphoproliferative lesions and peripheral blood lymphocytes (PBL) from 7 patients with primary SS, in PBL from 6 primary SS patients with no associated lymphoproliferative disorder, and in activated PBL from 2 healthy controls. T cell clonal expansion was investigated in 10 Vβ families (i.e., the most expanded ones and those previously implicated in SS pathogenesis) by single strand conformation polymorphism (SSCP) analysis. Frozen sections from parotid gland specimens were tested by immunohistochemistry for the expansion of selected Vβ families. Viral infection within the parotid lesions and serum autoantibody response were also studied. Results. An unrestricted Vβ pattern was observed. The most widely expressed Vβ family in parotid lesions was Vβ2, and Vβ immunohistochemistry results were concordant with Vβ mRNA findings. A similar pattern was observed in PBL, although the Vβ2 family was expressed at lower levels. The parotid/PBL ratio was occasionally > 1.8-2.0 (indicative of local Vβ overexpression) in different Vβ families. T cell expansion proved to be largely polyclonal by SSCP analysis, and scattered T cell clonotypes were detected within different Vβ families, with a different pattern from patient to patient. Conclusion. Our observations in SS related lymphoproliferative lesions largely reflect previous evidence in fully benign lesions. The pathogenetic events involved in autoimmune benign lesions in SS may then persist and play a role in SS related lymphoproliferative disorders. The link between the observed TCR-Vβ repertoire and specific local triggering (auto)antigens remains to be elucidated.
KW - Parotid
KW - Sjogren's syndrome
KW - T cell receptor lymphoma
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M3 - Article
C2 - 10332975
AN - SCOPUS:6544282757
VL - 26
SP - 1101
EP - 1109
JO - Journal of Rheumatology
JF - Journal of Rheumatology
SN - 0315-162X
IS - 5
ER -