T cell receptor repertoire in B cell lymphoproliferative lesions in primary Sjogren's syndrome

Barbara Pivetta, Salvatore De Vita, Gianfranco Ferraccioli, Valli De Re, Annunziata Gloghini, Alessandra Marzotto, Giuseppe Caruso, Riccardo Dolcetti, Ettore Bartoli, Antonino Carbone, Mauro Boiocchi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective. Studies have analyzed T cell receptor (TCR)-Vβ in benign, minor salivary or lacrimal gland, or kidney lesions in Sjogren's syndrome (SS). We investigated SS related lymphoproliferative lesions. Methods. By 'family' reverse transcriptase polymerase chain reaction, we studied the expression of 20 different TCR-Vβ families in parotid lymphoproliferative lesions and peripheral blood lymphocytes (PBL) from 7 patients with primary SS, in PBL from 6 primary SS patients with no associated lymphoproliferative disorder, and in activated PBL from 2 healthy controls. T cell clonal expansion was investigated in 10 Vβ families (i.e., the most expanded ones and those previously implicated in SS pathogenesis) by single strand conformation polymorphism (SSCP) analysis. Frozen sections from parotid gland specimens were tested by immunohistochemistry for the expansion of selected Vβ families. Viral infection within the parotid lesions and serum autoantibody response were also studied. Results. An unrestricted Vβ pattern was observed. The most widely expressed Vβ family in parotid lesions was Vβ2, and Vβ immunohistochemistry results were concordant with Vβ mRNA findings. A similar pattern was observed in PBL, although the Vβ2 family was expressed at lower levels. The parotid/PBL ratio was occasionally > 1.8-2.0 (indicative of local Vβ overexpression) in different Vβ families. T cell expansion proved to be largely polyclonal by SSCP analysis, and scattered T cell clonotypes were detected within different Vβ families, with a different pattern from patient to patient. Conclusion. Our observations in SS related lymphoproliferative lesions largely reflect previous evidence in fully benign lesions. The pathogenetic events involved in autoimmune benign lesions in SS may then persist and play a role in SS related lymphoproliferative disorders. The link between the observed TCR-Vβ repertoire and specific local triggering (auto)antigens remains to be elucidated.

Original languageEnglish
Pages (from-to)1101-1109
Number of pages9
JournalJournal of Rheumatology
Volume26
Issue number5
Publication statusPublished - 1999

Keywords

  • Parotid
  • Sjogren's syndrome
  • T cell receptor lymphoma

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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