T cell receptor (TCR) gene transfer with lentiviral vectors allows efficient redirection of tumor specificity in naive and memory T cells without prior stimulation of endogenous TCR

Paola Circosta, Luisa Granziero, Antonia Follenzi, Elisa Vigna, Stefania Stella, Antonella Vallario, Angela Rita Elia, Loretta Gammaitoni, Katiuscia Vitaggio, Francesca Orso, Massimo Geuna, Dario Sangiolo, Maja Todorovic, Claudia Giachino, Alessandro Cignetti

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We investigated the possibility of introducing exogenous T cell receptor (TCR) genes into T cells by lentiviral transduction, without prior stimulation of endogenous TCR with anti-CD3. TCR transfer is used to impose tumor antigen specificity on recipient T cells, but sustained activation required for retroviral transduction may affect the clinical efficacy of engineered T cells. Cytokine stimulation makes T cells susceptible to lentiviral transduction in the absence of TCR triggering, but this advantage has never been exploited for TCR transfer. Autoimmune diseases are a source of high-affinity TCRs specific for self/tumor antigens. We selected, from a patient with vitiligo, a Mart1-specific TCR based on intrinsic interchain pairing properties and functional avidity. After lentiviral transduction of human peripheral blood mononuclear cells, preferential pairing of exogenous α and β chains was observed, together with effective recognition of Mart1+ melanoma cells. We tested transduction efficiency on various T cell subsets prestimulated with interleukin (IL)-2, IL-7, IL-15, and IL-21 (alone or in combination). Both naive and unfractionated CD8+ T cells could be transduced without requiring endogenous TCR triggering. IL-7 plus IL-15 was the most powerful combination, allowing high levels of transgene expression without inducing T cell differentiation (34±5% Mart1-TCR+ cells in naive CD8 + and 16±6% in unfractionated CD8+). Cytokine-prestimulated, Mart1-redirected naive and unfractionated CD8 + cells expanded better than CD3-CD28-prestimulated counterparts in response to both peptide-pulsed antigen-presenting cells and Mart1+ melanoma cells. This strategy allows the generation of tumor-specific T cells encompassing truly naive T cells, endowed with an intact proliferative potential and a preserved differentiation stage.

Original languageEnglish
Pages (from-to)1576-1588
Number of pages13
JournalHuman Gene Therapy
Volume20
Issue number12
DOIs
Publication statusPublished - Dec 1 2009

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T-Cell Receptor Genes
T-Cell Antigen Receptor
T-Lymphocytes
Neoplasms
Interleukin-15
Interleukin-7
Neoplasm Antigens
Melanoma
Cytokines
Vitiligo
Autoantigens
T-Lymphocyte Subsets
Antigen-Presenting Cells
Transgenes
Autoimmune Diseases
Interleukin-2
Cell Differentiation
Blood Cells
Peptides

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

T cell receptor (TCR) gene transfer with lentiviral vectors allows efficient redirection of tumor specificity in naive and memory T cells without prior stimulation of endogenous TCR. / Circosta, Paola; Granziero, Luisa; Follenzi, Antonia; Vigna, Elisa; Stella, Stefania; Vallario, Antonella; Elia, Angela Rita; Gammaitoni, Loretta; Vitaggio, Katiuscia; Orso, Francesca; Geuna, Massimo; Sangiolo, Dario; Todorovic, Maja; Giachino, Claudia; Cignetti, Alessandro.

In: Human Gene Therapy, Vol. 20, No. 12, 01.12.2009, p. 1576-1588.

Research output: Contribution to journalArticle

Circosta, Paola ; Granziero, Luisa ; Follenzi, Antonia ; Vigna, Elisa ; Stella, Stefania ; Vallario, Antonella ; Elia, Angela Rita ; Gammaitoni, Loretta ; Vitaggio, Katiuscia ; Orso, Francesca ; Geuna, Massimo ; Sangiolo, Dario ; Todorovic, Maja ; Giachino, Claudia ; Cignetti, Alessandro. / T cell receptor (TCR) gene transfer with lentiviral vectors allows efficient redirection of tumor specificity in naive and memory T cells without prior stimulation of endogenous TCR. In: Human Gene Therapy. 2009 ; Vol. 20, No. 12. pp. 1576-1588.
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AU - Vigna, Elisa

AU - Stella, Stefania

AU - Vallario, Antonella

AU - Elia, Angela Rita

AU - Gammaitoni, Loretta

AU - Vitaggio, Katiuscia

AU - Orso, Francesca

AU - Geuna, Massimo

AU - Sangiolo, Dario

AU - Todorovic, Maja

AU - Giachino, Claudia

AU - Cignetti, Alessandro

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