T-cell receptor Vδ gene usage by tumour reactive γδ T lymphocytes infiltrating human lung cancer

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Abstract

In seven human adenocarcinomas and a non-neoplastic granulomatous disease of the lung, γδ+ infiltrating lymphocytes (TIL) could be isolated and selectively expanded in vitro upon culture in interleukin-2 (IL-2), without any additional stimuli, indicating a prior activation in vivo. In most cases γδ TIL were predominantly Vδ1+, despite a normal Vδ2:Vδ1 ratio in paired peripheral blood lymphocytes, suggesting a possible expansion of this subset in response to localized antigens/superantigens. Moreover, in five patients it was possible to identify a Vδ1- Vδ2- TIL population which by polymerase chain reaction (PCR) analysis was shown to be heterogeneous as Vδ gene usage, inclusive of Vδ 3,4,5,6,7 and 8. Of note, these Vδ regions have not been found in peripheral blood so far. Finally, in all cases, γδ TIL displayed killing activity of the autologous tumour, which appeared to be more restricted in the case of Vδ1+ cells. Altogether, these findings suggest a preferential expansion, at the tumour site, of Vδ1+ cells and of cells expressing Vδ genes other than Vδ2.

Original languageEnglish
Pages (from-to)234-239
Number of pages6
JournalImmunology
Volume81
Issue number2
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Immunology

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