T-cell recognition of chemicals, protein allergens and drugs: Towards the development of in vitro assays

Stefan F. Martin, Philipp R. Esser, Sonja Schmucker, Lisa Dietz, Dean J. Naisbitt, B. Kevin Park, Marc Vocanson, Jean Francois Nicolas, Monika Keller, Werner J. Pichler, Matthias Peiser, Andreas Luch, Reinhard Wanner, Enrico Maggi, Andrea Cavani, Thomas Rustemeyer, Anne Richter, Hermann Josef Thierse, Federica Sallusto

Research output: Contribution to journalArticle

Abstract

Chemicals can elicit T-cell-mediated diseases such as allergic contact dermatitis and adverse drug reactions. Therefore, testing of chemicals, drugs and protein allergens for hazard identification and risk assessment is essential in regulatory toxicology. The seventh amendment of the EU Cosmetics Directive now prohibits the testing of cosmetic ingredients in mice, guinea pigs and other animal species to assess their sensitizing potential. In addition, the EU Chemicals Directive REACh requires the retesting of more than 30,000 chemicals for different toxicological endpoints, including sensitization, requiring vast numbers of animals. Therefore, alternative methods are urgently needed to eventually replace animal testing. Here, we summarize the outcome of an expert meeting in Rome on 7 November 2009 on the development of T-cell-based in vitro assays as tools in immunotoxicology to identify hazardous chemicals and drugs. In addition, we provide an overview of the development of the field over the last two decades.

Original languageEnglish
Pages (from-to)4171-4184
Number of pages14
JournalCellular and Molecular Life Sciences
Volume67
Issue number24
DOIs
Publication statusPublished - Dec 2010

Keywords

  • Allergy
  • Bioassay
  • Contact dermatitis
  • Drug hypersensitivity
  • Immunotoxicology
  • In vitro alternatives
  • Non-animal testing
  • T cell

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Martin, S. F., Esser, P. R., Schmucker, S., Dietz, L., Naisbitt, D. J., Park, B. K., Vocanson, M., Nicolas, J. F., Keller, M., Pichler, W. J., Peiser, M., Luch, A., Wanner, R., Maggi, E., Cavani, A., Rustemeyer, T., Richter, A., Thierse, H. J., & Sallusto, F. (2010). T-cell recognition of chemicals, protein allergens and drugs: Towards the development of in vitro assays. Cellular and Molecular Life Sciences, 67(24), 4171-4184. https://doi.org/10.1007/s00018-010-0495-3