TY - JOUR
T1 - T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis
AU - Cavalcanti, Catarina Addobbati Jordão
AU - Germoglio, Vanessa
AU - de Azevêdo Silva, Jaqueline
AU - Glesse, Nadine
AU - Vianna, Priscila
AU - Cechim, Giovana
AU - Monticielo, Odirlei Andre
AU - Xavier, Ricardo Machado
AU - Brenol, João Carlos Tavares
AU - Brenol, Claiton Viegas
AU - Fragoso, Thiago Sotero
AU - Barbosa, Alexandre Domingues
AU - Duarte, Ângela Luiza Branco Pinto
AU - Oliveira, Renê Donizeti Ribeiro
AU - Louzada-Júnior, Paulo
AU - Donadi, Eduardo Antônio
AU - Chies, José Artur Bogo
AU - Crovella, Sergio
AU - Sandrin-Garcia, Paula
N1 - Funding Information:
This work was supported by the following Brazilian funding agencies: CAPES (Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior), CNPq (Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico), FAPESP (Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo), FAPERGS (Funda??o de Amparo ? Pesquisa do Estado do Rio Grande do Sul), FACEPE (Funda??o de Amparo ? Ci?ncia e Tecnologia de Pernambuco), and FIPE/HCPA (Fundo de Incentivo ? Pesquisa e Eventos do Hospital de Cl?nicas de Porto Alegre).
Publisher Copyright:
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/2/17
Y1 - 2020/2/17
N2 - The aim of the present study was to evaluate the impact of SEMA4A genetic variants on expression of sema4A protein and its relation to autoimmunity development in Systemic Lupus Erythematosus and Rheumatoid Arthritis patients. A total of 541 SLE patients, 390 RA patients and 607 healthy individuals were genotyped. We also assessed SEMA4A mRNA expression from whole blood cells and the in vitro protein production from resting and activated T lymphocytes as well as mature dendritic cells from healthy individuals stratified according to their genotypes for SLE/RA associated SEMA4A variants. Our results showed that T/T genotype for rs3738581 SNP is associated with both RA and SLE development (p =.000053, OR = 2.35; p =.0019, OR = 2.07, respectively; statistical power = 100%) and also to an increased in vitro sema4A production in active T lymphocytes. Our findings are indicative of a T cell-specific upregulation of sema4A in the presence of T/T genotype, being a risk factor for SLE and RA.
AB - The aim of the present study was to evaluate the impact of SEMA4A genetic variants on expression of sema4A protein and its relation to autoimmunity development in Systemic Lupus Erythematosus and Rheumatoid Arthritis patients. A total of 541 SLE patients, 390 RA patients and 607 healthy individuals were genotyped. We also assessed SEMA4A mRNA expression from whole blood cells and the in vitro protein production from resting and activated T lymphocytes as well as mature dendritic cells from healthy individuals stratified according to their genotypes for SLE/RA associated SEMA4A variants. Our results showed that T/T genotype for rs3738581 SNP is associated with both RA and SLE development (p =.000053, OR = 2.35; p =.0019, OR = 2.07, respectively; statistical power = 100%) and also to an increased in vitro sema4A production in active T lymphocytes. Our findings are indicative of a T cell-specific upregulation of sema4A in the presence of T/T genotype, being a risk factor for SLE and RA.
KW - polymorphism
KW - rheumatoid arthritis
KW - SEMA4A
KW - systemic lupus erythematosus
KW - T cells
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U2 - 10.1080/08916934.2019.1704273
DO - 10.1080/08916934.2019.1704273
M3 - Article
C2 - 31876207
AN - SCOPUS:85077361280
VL - 53
SP - 65
EP - 70
JO - Autoimmunity
JF - Autoimmunity
SN - 0891-6934
IS - 2
ER -