Abstract
The supernatant of T(E)+ lymphocytes isolated by density gradient from a lympho-epithelial thymoma contained a biochemical variant of T cell growth factor (IL-2). This protein showed two peaks with an apparent molecular weight of 18 and 28 kd by Sephadex G 100 chromatography. Further purification was achieved by DEAE Sephacel and CM Sepharose ion-exchange chromatography. By isoelectric focusing the isoelectric point was found to differ from that of normal IL-2. Based on these results, the IL-2 variant appears to be a new kind of lymphokine distinguishable from normal IL-2 and similar to a TCGF (L-TCGF peak II) purified from the malignant T cell clone HUT 102.
Original language | English |
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Pages (from-to) | 251-263 |
Number of pages | 13 |
Journal | Lymphokine Research |
Volume | 4 |
Issue number | 3 |
Publication status | Published - 1985 |
ASJC Scopus subject areas
- Immunology
- Hematology