T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients

A. Di Stefano, G. Caramori, I. Gnemmi, M. Contoli, C. Vicari, A. Capelli, F. Magno, S. E. D'Anna, A. Zanini, P. Brun, P. Casolari, K. F. Chung, P. J. Barnes, A. Papi, I. Adcock, B. Balbi

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Abstract

There are increased numbers of activated T lymphocytes in the bronchial mucosa of stable chronic obstructive pulmonary disease (COPD) patients. T helper type 17 (Th17) cells release interleukin (IL)-17 as their effector cytokine under the control of IL-22 and IL-23. Furthermore, Th17 numbers are increased in some chronic inflammatory conditions. To investigate the expression of interleukin (IL)-17A, IL-17F, IL-21, IL-22 and IL-23 and of retinoic orphan receptor RORC2, a marker of Th17 cells, in bronchial biopsies from patients with stable COPD of different severity compared with age-matched control subjects. The expression of IL-17A, IL-17F, IL-21, IL-22, IL-23 and RORC2 was measured in the bronchial mucosa using immunohistochemistry and/or quantitative polymerase chain reaction. The number of IL-22 + and IL-23 + immunoreactive cells is increased in the bronchial epithelium of stable COPD compared with control groups. In addition, the number of IL-17A + and IL-22 + immunoreactive cells is increased in the bronchial submucosa of stable COPD compared with control non-smokers. In all smokers, with and without disease, and in patients with COPD alone, the number of IL-22 + cells correlated significantly with the number of both CD4 + and CD8 + cells in the bronchial mucosa. RORC2 mRNA expression in the bronchial mucosa was not significantly different between smokers with normal lung function and COPD. Further, we report that endothelial cells express high levels of IL-17A and IL-22. Increased expression of the Th17-related cytokines IL-17A, IL-22 and IL-23 in COPD patients may reflect their involvement, and that of specific IL-17-producing cells, in driving the chronic inflammation seen in COPD.

Original languageEnglish
Pages (from-to)316-324
Number of pages9
JournalClinical and Experimental Immunology
Volume157
Issue number2
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Interleukin-17
Chronic Obstructive Pulmonary Disease
Mucous Membrane
Interleukin-23
Cytokines
Th17 Cells
interleukin-22
Epithelium
Endothelial Cells
Immunohistochemistry
Inflammation
T-Lymphocytes
Biopsy
Polymerase Chain Reaction
Lung
Control Groups
Messenger RNA

Keywords

  • Autoimmunity
  • Bronchial biopsies
  • Emphysema
  • Neutrophils
  • Pathology

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients. / Di Stefano, A.; Caramori, G.; Gnemmi, I.; Contoli, M.; Vicari, C.; Capelli, A.; Magno, F.; D'Anna, S. E.; Zanini, A.; Brun, P.; Casolari, P.; Chung, K. F.; Barnes, P. J.; Papi, A.; Adcock, I.; Balbi, B.

In: Clinical and Experimental Immunology, Vol. 157, No. 2, 08.2009, p. 316-324.

Research output: Contribution to journalArticle

Di Stefano, A. ; Caramori, G. ; Gnemmi, I. ; Contoli, M. ; Vicari, C. ; Capelli, A. ; Magno, F. ; D'Anna, S. E. ; Zanini, A. ; Brun, P. ; Casolari, P. ; Chung, K. F. ; Barnes, P. J. ; Papi, A. ; Adcock, I. ; Balbi, B. / T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients. In: Clinical and Experimental Immunology. 2009 ; Vol. 157, No. 2. pp. 316-324.
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abstract = "There are increased numbers of activated T lymphocytes in the bronchial mucosa of stable chronic obstructive pulmonary disease (COPD) patients. T helper type 17 (Th17) cells release interleukin (IL)-17 as their effector cytokine under the control of IL-22 and IL-23. Furthermore, Th17 numbers are increased in some chronic inflammatory conditions. To investigate the expression of interleukin (IL)-17A, IL-17F, IL-21, IL-22 and IL-23 and of retinoic orphan receptor RORC2, a marker of Th17 cells, in bronchial biopsies from patients with stable COPD of different severity compared with age-matched control subjects. The expression of IL-17A, IL-17F, IL-21, IL-22, IL-23 and RORC2 was measured in the bronchial mucosa using immunohistochemistry and/or quantitative polymerase chain reaction. The number of IL-22 + and IL-23 + immunoreactive cells is increased in the bronchial epithelium of stable COPD compared with control groups. In addition, the number of IL-17A + and IL-22 + immunoreactive cells is increased in the bronchial submucosa of stable COPD compared with control non-smokers. In all smokers, with and without disease, and in patients with COPD alone, the number of IL-22 + cells correlated significantly with the number of both CD4 + and CD8 + cells in the bronchial mucosa. RORC2 mRNA expression in the bronchial mucosa was not significantly different between smokers with normal lung function and COPD. Further, we report that endothelial cells express high levels of IL-17A and IL-22. Increased expression of the Th17-related cytokines IL-17A, IL-22 and IL-23 in COPD patients may reflect their involvement, and that of specific IL-17-producing cells, in driving the chronic inflammation seen in COPD.",
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AU - Caramori, G.

AU - Gnemmi, I.

AU - Contoli, M.

AU - Vicari, C.

AU - Capelli, A.

AU - Magno, F.

AU - D'Anna, S. E.

AU - Zanini, A.

AU - Brun, P.

AU - Casolari, P.

AU - Chung, K. F.

AU - Barnes, P. J.

AU - Papi, A.

AU - Adcock, I.

AU - Balbi, B.

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